SUMO modification modulates the transrepression activity of PLZF

被引：23

作者：

Chao, Ting-Ting

Chang, Che-Chang

Shih, Hsiu-Ming

机构：

[1] Acad Sinica, Inst Biomed Sci, Taipei 11529, Taiwan

[2] Natl Def Med Ctr, Grad Inst Life Sci, Taipei, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 358卷 / 02期

关键词：

SUMO modification; PLZF; transcriptional repression; nuclear body;

D O I：

10.1016/j.bbrc.2007.04.157

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression. (C) 2007 Elsevier Inc. All rights reserved.

引用

收藏

页码：475 / 482

页数：8

相关论文

共 33 条

[1] Daxx mediates the small ubiquitin-like modifier-dependent transcriptional repression of Smad [J].

Chang, CC ;

Lin, DY ;

Fang, HI ;

Chen, RH ;

Shih, HM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (11) :10164-10173

[2] Efficacy and safety analyses of a recombinant human immunodeficiency virus type 1 derived vector system [J].

Chang, LJ ;

Urlacher, V ;

Iwakuma, T ;

Cui, Y ;

Zucali, J .

GENE THERAPY, 1999, 6 (05) :715-728

[3] Histone deacetylase associated with mSin3A mediates repression by the acute promyelocytic leukemia-associated PLZF protein [J].

David, G ;

Alland, L ;

Hong, SH ;

Wong, CW ;

DePinho, RA ;

Dejean, A .

ONCOGENE, 1998, 16 (19) :2549-2556

[4] Colocalization and heteromerization between the two human oncogene POZ/zinc finger proteins, LAZ3 (BCL6) and PLZF [J].

Dhordain, P ;

Albagli, O ;

Honore, N ;

Guidez, F ;

Lantoine, D ;

Schmid, M ;

De The, H ;

Zelent, A ;

Koken, MHM .

ONCOGENE, 2000, 19 (54) :6240-6250

[5] Amino-terminal protein-protein interaction motif (POZ-domain) is responsible for activities of the promyelocytic leukemia zinc finger retinoic acid receptor alpha fusion protein [J].

Dong, S ;

Zhu, J ;

Reid, A ;

Strutt, P ;

Guidez, F ;

Zhong, HJ ;

Wang, ZY ;

Licht, J ;

Waxman, S ;

Chomienne, C ;

Chen, Z ;

Zelent, A ;

Chen, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (08) :3624-3629

[6] Something about SUMO inhibits transcription [J].

Gill, G .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2005, 15 (05) :536-541

[7] p300 transcriptional repression is mediated by SUMO modification [J].

Girdwood, D ;

Bumpass, D ;

Vaughan, OA ;

Thain, A ;

Anderson, LA ;

Snowden, AW ;

Garcia-Wilson, E ;

Perkins, ND ;

Hay, RT .

MOLECULAR CELL, 2003, 11 (04) :1043-1054

[8] Fusion proteins of the retinoic acid receptor-α recruit histone deacetylase in promyelocytic leukaemia [J].

Grignani, F ;

De Matteis, S ;

Nervi, C ;

Tomassoni, L ;

Gelmetti, V ;

Cioce, M ;

Fanelli, M ;

Ruthardt, M ;

Ferrara, FF ;

Zamir, I ;

Seiser, C ;

Grignani, F ;

Lazar, MA ;

Minucci, S ;

Pelicci, PG .

NATURE, 1998, 391 (6669) :815-818

[9] Reduced retinoic acid-sensitivities of nuclear receptor corepressor binding to PML- and PLZF-RARα underlie molecular pathogenesis and treatment of acute promyelocytic leukemia [J].

Guidez, F ;

Ivins, S ;

Zhu, J ;

Söderström, M ;

Waxman, S ;

Zelent, A .

BLOOD, 1998, 91 (08) :2634-2642

[10] SUMO: A history of modification [J].

Hay, RT .

MOLECULAR CELL, 2005, 18 (01) :1-12

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