Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)

被引：1

作者：

Watase Kunii, Gabriela Kinue ^{[1
]}

Falcone, Rossana ^{[1
]}

Clementino, Leandro da Costa ^{[1
]}

da Rosa, Joao Aristeu ^{[1
]}

Nascimento, Juliana Damieli ^{[2
]}

Belintani, Tiago ^{[2
]}

de Oliveira, Jader ^{[3
]}

Ribeiro, Aline Rimoldi ^{[4
]}

机构：

[1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Araraquara, SP, Brazil

[2] Univ Estadual Campinas, Dept Biol Anim, Campinas, SP, Brazil

[3] Univ Sao Paulo, Dept Epidemiol, Sao Paulo, SP, Brazil

[4] Univ Estadual Campinas, Dept Genet Evolucao Microbiol & Imunol, Campinas, SP, Brazil

来源：

REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL | 2022年 / 55卷

基金：

巴西圣保罗研究基金会;

关键词：

Chagas disease; Trypanosoma cruzi; Epidemiology; Vector biology; CHAGAS;

D O I：

10.1590/0037-8682-0521-2021

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.

引用

页数：4

共 15 条

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