Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design

被引：3

作者：

Li, Wenpan ^{[1
]}

Jing, Shasha ^{[1
]}

Xin, Xiu ^{[1
]}

Zhang, Xirui ^{[1
]}

Chen, Kang ^{[1
]}

Chen, Dawei ^{[1
]}

Hu, Haiyang ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Dept Pharmaceut, Sch Pharm, 103 Wenhua Rd, Shenyang 110016, Peoples R China

来源：

ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2017年 / 12卷 / 02期

关键词：

CaP nanoparticles; pDNA; Reverse microemulsion method; Box-Behnken design; Transfection and expression; NONVIRAL GENE DELIVERY; CALCIUM-PHOSPHATE; HYDROXYAPATITE; TRANSFECTION; SIZE; EFFICIENCY; CELLS;

D O I：

10.1016/j.ajps.2016.09.006

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box-Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles were found to be 60.23 +/- 4.72 nm, polydispersity index was 0.252 and pDNA encapsulate efficiency was more than 90%. The optimized CaP/pDNA nanoparticles have pH sensitivity and biocompatibility. Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency. (C) 2017 Shenyang Pharmaceutical University. Production and hosting by Elsevier B.V.

引用

页码：179 / 186

页数：8

共 6 条

[1] Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design
Wenpan Li
Shasha Jing
Xiu Xin
Xirui Zhang
Kang Chen
Dawei Chen
Haiyang Hu
Asian Journal of Pharmaceutical Sciences, 2017, 12 (02) : 179 - 186
[2] Preparation of Calcium Phosphate/pDNA Nanoparticles for Exogenous Gene Delivery by Co-Precipitation Method: Optimization of Formulation Variables Using Box-Behnken Design
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Zhang, Xirui
Jing, Shasha
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Chen, Kang
Chen, Dawei
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JOURNAL OF PHARMACEUTICAL SCIENCES, 2017, 106 (08) : 2053 - 2059
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[6] Process optimization for the preparation of oligomycin-loaded folate-conjugated chitosan nanoparticles as a tumor-targeted drug delivery system using a two-level factorial design method
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