Comparison of the cobas Human Immunodeficiency Virus 1 (HIV-1) Test Using the cobas 4800 System With COBAS AmpliPrep/COBAS TaqMan HIV-1 Test and Abbott RealTime HIV-1 Assay and Performance Evaluation of cobas HIV-1

被引：2

作者：

Shin, Kyung-Hwa ^{[1
,2
]}

Lee, Hyun-Ji ^{[3
]}

Lee, Jin Hyeon ^{[1
,2
]}

Chang, Chulhun L. ^{[3
]}

Kim, Hyung-Hoi ^{[1
,2
,4
]}

机构：

[1] Pusan Natl Univ Hosp, Dept Lab Med, Busan, South Korea

[2] Pusan Natl Univ Hosp, Biomed Res Inst, Busan, South Korea

[3] Pusan Natl Univ, Dept Lab Med, Yangsan Hosp, Yangsan, South Korea

[4] Pusan Natl Univ, Lab Med & BioMed Informat Unit, Sch Med, Busan, South Korea

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2019年 / 152卷 / 05期

关键词：

Human immunodeficiency virus; RNA quantification; Correlation analysis; VIRAL LOAD; GENETIC DIVERSITY; QUANTIFICATION; PLASMA;

D O I：

10.1093/ajcp/aqz075

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Objectives: To compare quantified human immunodeficiency virus type 1 (HIV-1) viral load quantified using the cobas HIV-1 test with that obtained using the CAP/CTM HIV-1 and Abbott RealTime HIV-1 tests and evaluate the performance of cobas HIV-1 using the cobas 4800 system. Methods: Clinical samples (n = 123) were quantitatively analyzed using the CAP/CTM HIV-1, Abbott RealTime HIV-1, and cobas HIV-1 tests, and the precision, linearity, and limit of detection of the cobas HIV-1 test were evaluated. Results: Comparable results were obtained by both methods: ([log CAP/CTM HIV-1 value] = 0.979 * [log cobas HIV-1 test value] + 0.034) and ([log Abbott RealTime HIV-1 value] = 0.985 * [log cobas HIV-1 test value] + 0.027). cobas HIV-1 test results for 89.4% and 93.5% were within 0.5 log(10) IU/mL of the CAP/CTM HIV-1 and Abbott RealTime HIV-1 results, respectively. Conclusions: The cobas HIV-1 test showed good performance, and its results correlated well with those of other two tests.

引用

页码：558 / 562

页数：5

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