Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

被引：3

作者：

Pei, Haiyun ^{[1
,2
,3
]}

Fu, Hsu-Yuan ^{[1
]}

Hirai, Hiroyuki ^{[4
]}

Cho, Dong Seong ^{[1
,2
]}

O'Brien, Timothy D. ^{[2
,6
]}

Dutton, James ^{[2
]}

Verfaillie, Catherine M. ^{[5
]}

Hu, Wei-Shou ^{[1
,2
]}

机构：

[1] Dept Chem Engn & Mat Sci, Minneapolis, MN USA

[2] Univ Minnesota, Stem Cell Inst, Minneapolis, MN USA

[3] Beijing Inst Transfus Med, Stem Cell & Regenerat Med Lab, Beijing, Peoples R China

[4] Univ Michigan, Dept Cardiac Surg, Ann Arbor, MI 48109 USA

[5] Katholieke Univ Leuven, Dept Dev & Regenerat, Leuven, Belgium

[6] Univ Minnesota, Vet Populat Med Dept, St Paul, MN 55108 USA

来源：

STEM CELL RESEARCH | 2017年 / 21卷

关键词：

D O I：

10.1016/j.scr.2017.04.010

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox(2), Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines. (C) 2017 The Authors. Published by Elsevier B.V.

引用

页码：132 / 136

页数：5

共 4 条

[1] Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells [J].