LESION SIMULATING DISEASE 1 and ENHANCED DISEASE SUSCEPTIBILITY 1 differentially regulate UV-C-induced photooxidative stress signalling and programmed cell death in Arabidopsis thaliana

被引：63

作者：

Wituszynska, Weronika ^{[1
,2
]}

Szechynska-Hebda, Magdalena ^{[1
,3
]}

Sobczak, Miroslaw ^{[2
]}

Rusaczonek, Anna ^{[1
]}

Kozlowska-Makulska, Anna ^{[1
]}

Witon, Damian ^{[1
]}

Karpinski, Stanislaw ^{[1
]}

机构：

[1] Warsaw Univ Life Sci SGGW, Dept Plant Genet Breeding & Biotechnol, Fac Hort Biotechnol & Landscape Architecture, PL-02776 Warsaw, Poland

[2] Warsaw Univ Life Sci SGGW, Fac Agr & Biol, Dept Biol, PL-02776 Warsaw, Poland

[3] Polish Acad Sci, Inst Plant Physiol, PL-30239 Krakow, Poland

来源：

PLANT CELL AND ENVIRONMENT | 2015年 / 38卷 / 02期

关键词：

abiotic stress; antioxidative enzymes; chlorophyll fluorescence; oxidative stress; transmission electron microscopy; THERMAL-ENERGY DISSIPATION; PHOTOSYSTEM-II; GENE-EXPRESSION; HYDROGEN-PEROXIDE; ULTRAVIOLET-B; SUPEROXIDE-DISMUTASE; BETA-CAROTENE; HIGHER-PLANTS; SALT STRESS; RADIATION;

D O I：

10.1111/pce.12288

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

As obligate photoautotrophs, plants are inevitably exposed to ultraviolet (UV) radiation. Because of stratospheric ozone depletion, UV has become more and more dangerous to the biosphere. Therefore, it is important to understand UV perception and signal transduction in plants. In the present study, we show that LESION SIMULATING DISEASE 1 (LSD1) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) are antagonistic regulators of UV-C-induced programmed cell death (PCD) in Arabidopsis thaliana. This regulatory dependence is manifested by a complex deregulation of photosynthesis, reactive oxygen species homeostasis, antioxidative enzyme activity and UV-responsive genes expression. We also prove that a UV-C radiation episode triggers apoptotic-like morphological changes within the mesophyll cells. Interestingly, chloroplasts are the first organelles that show features of UV-C-induced damage, which may indicate their primary role in PCD development. Moreover, we show that Arabidopsis Bax inhibitor 1 (AtBI1), which has been described as a negative regulator of plant PCD, is involved in LSD1-dependent cell death in response to UV-C. Our results imply that LSD1 and EDS1 regulate processes extinguishing excessive energy, reactive oxygen species formation and subsequent PCD in response to different stresses related to impaired electron transport. Because of stratospheric ozone depletion, UV has become more and more dangerous to the biosphere. Therefore, it is important to understand UV perception and signal transduction in plants. In the present study we show that LESION SIMULATING DISEASE 1 (LSD1) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) are antagonistic regulators of UV-C induced programmed cell death (PCD) in Arabidopsis thaliana. Moreover, we demonstrate that chloroplasts are the first organelles that show features of UV-C induced damage, which may indicate their primary role in PCD development.

引用

页码：315 / 330

页数：16

共 95 条

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