Oscillations in supercoiling drive circadian gene expression in cyanobacteria

被引：130

作者：

Vijayan, Vikram ^{[1
,2
,3
]}

Zuzow, Rick ^{[1
,2
]}

O'Shea, Erin K. ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, Harvard Fac Arts & Sci, Ctr Syst Biol, Howard Hughes Med Inst,Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

[3] Harvard Univ, Grad Program Syst Biol, Cambridge, MA 02138 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 52期

基金：

美国国家科学基金会;

关键词：

circadian clock; gene expression; supercoiling; cyanobacteria; KAIC PHOSPHORYLATION; ESCHERICHIA-COLI; DNA GYRASE; RHYTHMS; LUCIFERASE; REPORTER; FEEDBACK;

D O I：

10.1073/pnas.0912673106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cyanobacterium Synechococcus elongatus PCC 7942 exhibits oscillations in mRNA transcript abundance with 24-h periodicity under continuous light conditions. The mechanism underlying these oscillations remains elusive-neither cis nor trans-factors controlling circadian gene expression phase have been identified. Here, we show that the topological status of the chromosome is highly correlated with circadian gene expression state. We also demonstrate that DNA sequence characteristics of genes that appear monotonically activated and monotonically repressed by chromosomal relaxation during the circadian cycle are similar to those of supercoiling-responsive genes in Escherichia coli. Furthermore, perturbation of superhelical status within the physiological range elicits global changes in gene expression similar to those that occur during the normal circadian cycle.

引用

页码：22564 / 22568

页数：5

共 37 条

[1] DNA supercoiling and osmoresistance in Bacillus subtilis 168 [J].