Isolation of cDNA encoding full-length rat (Rattus norvegicus) poly(ADP-ribose) polymerase

被引：0
作者：
Beneke, S [1 ]
Meyer, R [1 ]
Bürkle, A [1 ]
机构：
[1] Deutsch Krebsforschungszentrum, Abt Tumorvirol 0610, Forschungsschwerpunkt Angew Tumorvirol, D-69120 Heidelberg, Germany
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1997年 / 43卷 / 04期
关键词：
poly(ADP-ribose) polymerase; PCR; mammalians; homology;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Poly(ADP-ribose) polymerase (EC 2.4.2.30) is a nuclear enzyme which binds to DNA breaks and then catalyzes the covalent modification of acceptor proteins with poly(ADP-ribose). Poly(ADP-ribose) polymerase activity contributes to the recovery of proliferating cells from DNA damage and to the maintenance of genomic stability, which may be mediated by effects on chromatin structure, DNA base-excision repair and cell cycle regulation. We established the complete cDNA sequence of rat poly(ADP-ribose) polymerase by RT-PCR and direct sequencing of amplification products and compared it with that of other mammalian species. The amino acid sequence homology is strikingly high. The best conserved regions are the known functional modules of poly(ADP-ribose) polymerase.
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页码：755 / 761
页数：7
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