The Drosophila Midkine/Pleiotrophin Homologues Miple1 and Miple2 Affect Adult Lifespan but Are Dispensable for Alk Signaling during Embryonic Gut Formation

被引：14

作者：

Hugosson, Fredrik ^{[1
]}

Sjogren, Camilla ^{[1
]}

Birve, Anna ^{[1
]}

Hedlund, Ludmilla ^{[1
]}

Eriksson, Therese ^{[1
]}

Palmer, Ruth H. ^{[1
,2
]}

机构：

[1] Umea Univ, Dept Mol Biol, Umea, Sweden

[2] Univ Gothenburg, Dept Med Biochem & Cell Biol, Gothenburg, Sweden

来源：

PLOS ONE | 2014年 / 9卷 / 11期

基金：

瑞典研究理事会;

关键词：

ANAPLASTIC LYMPHOMA KINASE; RECEPTOR TYROSINE KINASE; HEPARIN-BINDING PROTEIN; ELEGANS DAUER FORMATION; ACID-RESPONSIVE GENE; MOLECULE HB-GAM; GROWTH-FACTOR; CAENORHABDITIS-ELEGANS; VISCERAL MUSCLE; MONOCLONAL-ANTIBODIES;

D O I：

10.1371/journal.pone.0112250

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Midkine (MDK) and Pleiotrophin (PTN) are small heparin-binding cytokines with closely related structures. The Drosophila genome harbours two genes encoding members of the MDK/PTN family of proteins, known as miple1 and miple2. We have investigated the role of Miple proteins in vivo, in particular with regard to their proposed role as ligands for the Alk receptor tyrosine kinase (RTK). Here we show that Miple proteins are neither required to drive Alk signaling during Drosophila embryogenesis, nor are they essential for development in the fruit fly. Additionally we show that neither MDK nor PTN can activate hALK in vivo when ectopically co-expressed in the fly. In conclusion, our data suggest that Alk is not activated by MDK/PTN related growth factors Miple1 and Miple 2 in vivo.

引用

页数：14

共 82 条

[1] Anterograde jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila [J].