HPLC Analysis of Mycolic Acids in Evaluation of Drug Susceptibility of Mycobacterium tuberculosis Strains - Comparison with Conventional Methods

被引：0
作者：
Walkiewcz, Renata [1 ]
Chazan, Ryszarda [1 ]
Grubek-Jaworska, Hanna [1 ]
机构：
[1] Med Univ Warsaw, Dept Internal Med Pneumol & Allergol, PL-02097 Warsaw, Poland
来源：
BIOCYBERNETICS AND BIOMEDICAL ENGINEERING | 2010年 / 30卷 / 02期
关键词：
HPLC; mycolic acids; drug susceptibility; M; tuberculosis; RIFAMPIN RESISTANCE; SYSTEM; ASSAY;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The commonly used phenotypic tests of M. tuberculosis drug susceptibility evaluate drug effects on quantity of colony forming units (CFU) on solid media or CO2 secretion/O-2 consumption by cultures in liquid media. These tests are not precise enough and need a long time for the growth of the bacilli, so new more accurate and rapid methods are highly needed. The aim of this study was to determine the utility of quantitative HPLC analysis of mycolic acids for drug susceptibility of M. tuberculosis. In 119/120 (99.16%) of the performed tests the HPLC methods showed excellent agreement with the conventional phenotypic tests. The quantitative HPLC analysis of mycolic acids is a fairly exact indicator of tubercle bacilli growth and may be used as a quick and reliable measure of their drug sensitivity.
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页码：3 / 14
页数：12
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