Determination of the binding constant between progesterone and its monoclonal antibody using affinity capillary electrophoresis

被引：0

作者：

Wang, QG

Wang, YM

Luo, GA ^{[1
]}

Yang, SB

机构：

[1] Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China

[2] Univ Hong Kong, Dept Obstet & Gynecol, Hong Kong, Hong Kong, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2000年 / 28卷 / 06期

关键词：

affinity capillary electrophoresis; laser induced fluorescence detection; progesterone; binding constant;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The binding constant (Kb) between fluorescein isothiocyanate labeled progesterone (FP) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis with laser induced fluorescence (LTF) detection. Free FP and the complexes were separated by capillary electrophoresis in 3 min after 90 min incubation. The running buffer was 50 mmol/L Tricine with pH 8.4, and separation voltage was 20 kV. The Kb was determined to be 4.4 x 10(7) L/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on the determination were also studied.

引用

页码：731 / 734

页数：4