Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging

被引：20

作者：

Kritchenkov, Ilya S. ^{[1
]}

Solomatina, Anastasia, I ^{[1
]}

Kozina, Daria O. ^{[1
]}

Porsev, Vitaly V. ^{[1
]}

Sokolov, Victor V. ^{[1
]}

Shirmanova, Marina, V ^{[2
]}

Lukina, Maria M. ^{[2
]}

Komarova, Anastasia D. ^{[2
]}

Shcheslayskiy, Vladislav, I ^{[2
,3
]}

Belyaeva, Tatiana N. ^{[4
]}

Litvinov, Ilia K. ^{[4
]}

Salova, Anna, V ^{[4
]}

Kornilova, Elena S. ^{[4
,5
]}

Kachkin, Daniel, V ^{[6
]}

Tunik, Sergey P. ^{[1
]}

机构：

[1] St Petersburg State Univ, Inst Chem, Univ Skii Av 26, St Petersburg 198504, Russia

[2] Privolzhskiy Res Med Univ, Inst Expt Oncol & Biomed Technol, Minin & Pozharsky Sq 10-1, Nizhnii Novgorod 603005, Russia

[3] Becker & Hickl GmbH, Nunsdorfer Ring 7-9, D-12277 Berlin, Germany

[4] Russian Acad Sci, Inst Cytol, Tikhoretsky Av 4, St Petersburg 194064, Russia

[5] Peter Great St Petersburg Polytech Univ, Inst Biomed Syst & Biotechnol, Khlopina Str 11, St Petersburg 194021, Russia

[6] St Petersburg State Univ, Fac Biol, Univ Skaya Emb 7-9, St Petersburg 199034, Russia

来源：

MOLECULES | 2021年 / 26卷 / 10期

基金：

俄罗斯科学基金会; 俄罗斯基础研究基金会;

关键词：

Ir(III) complexes; NIR emitters; phosphorescence lifetime imaging; oxygen sensing; IRIDIUM(III) POLYPYRIDINE COMPLEXES; IN-VIVO; CYCLOMETALATED PLATINUM(II); PHOTOPHYSICAL PROPERTIES; LUMINESCENT RHENIUM(I); EMISSIVE BEHAVIOR; MITOCHONDRIA; PROBES; CELLS; NANOPARTICLES;

D O I：

10.3390/molecules26102898

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry. We have designed and synthetized four novel iridium [Ir((NC)-C-boolean AND)(2)((NN)-N-boolean AND)](+) complexes ((NC)-C-boolean AND-benzothienyl-phenanthridine based cyclometalated ligand; (NN)-N-boolean AND-pyridin-phenanthroimidazol diimine chelate), decorated with oligo(ethylene glycol) groups to impart these emitters' solubility in aqueous media, biocompatibility, and to shield them from interaction with bio-environment. These substances were fully characterized using NMR spectroscopy and ESI mass-spectrometry. The complexes exhibited excitation close to the biological "window of transparency", NIR emission at 730 nm, and quantum yields up to 12% in water. The compounds with higher degree of the chromophore shielding possess low toxicity, bleaching stability, absence of sensitivity to variations of pH, serum, and complex concentrations. The properties of these probes as oxygen sensors for biological systems have been studied by using phosphorescence lifetime imaging experiments in different cell cultures. The results showed essential lifetime response onto variations in oxygen concentration (2.0-2.3 mu s under normoxia and 2.8-3.0 mu s under hypoxia conditions) in complete agreement with the calibration curves obtained "in cuvette". The data obtained indicate that these emitters can be used as semi-quantitative oxygen sensors in biological systems.

引用

页数：22

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