Orexin-A inhibits capsaicin-induced changes in cyclooxygenase-2 and brain-derived neurotrophic factor expression in trigeminal nucleus caudalis of rats

被引：12
作者：
Kooshki, Razieh [1 ]
Abbasnejad, Mehdi [1 ]
Mahani, Saeed Esmaeili [1 ]
Raoof, Maryam [2 ,3 ]
Aghtaei, Mohammad Mehdi Moeini [4 ]
Dabiri, Shahriar [4 ]
机构：
[1] Shahid Bahonar Univ Kerman, Fac Sci, Dept Biol, Kerman, Iran
[2] Kerman Univ Med Sci, Endodontol Res Ctr, Kerman 7616913555, Iran
[3] Kerman Univ Med Sci, Inst Neuropharmacol, Neurosci Res Ctr, Lab Mol Neurosci, Kerman, Iran
[4] Afzalipour Kerman Univ Med Sci, Pathol & Stem Cell Res Ctr, Dept Pathol, Kerman, Iran
来源：
KOREAN JOURNAL OF PAIN | 2018年 / 31卷 / 03期
关键词：
Brain-derived neurotrophic factor (BDRF); Capsaicin; Cyclooxygenase 2 (COX 2); Orexin-A; Orexin receptor antagonists; Orofacial pain; Rats; Pain measurement; Pain perception; Trigeminal caudal nucleus; Trigeminal neuralgia; OXIDATIVE STRESS; RECEPTOR; PAIN; ACTIVATION; GANGLION; BDNF; HYPERALGESIA; NEURONS; MEMORY; CGRP;
D O I：
10.3344/kjp.2018.31.3.174
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
Background: The trigeminal nucleus caudalis (Vc) is a primary central site for trigeminal transmitting. Noxious stimulation of the trigeminal nociceptors alters the central synaptic releases and neural expression of some inflammatory and trophic agents. Orexin-A and the orexin 1 receptor (OX1R) are expressed in pain pathways including trigeminal pain transmission. However, the the mechanism(s) underling orexin-A effects on trigeminal pain modulation have not been fully clarified. Methods: Trigeminal pain was induced by subcutaneous injection of capsaicin in the upper lip in rats. The effect of trigeminal pain on cyclooxygenase-2 (COX-2) and brain-derived neurotrophic factor (BDNF) expression in the Vc of animals was determined by immunofluorescence. Subsequently, OX1R agonist (orexin-A) and antagonist (SB-334867-A) was administrated in the Vc to investigate the possible roles of the Vc OX1R on changes in COX-2 and BDNF levels following pain induction. Results: The data indicated an increase in COX-2 and decrease in BDNF immuno-reactivity in the Vc of capsaicin, and capsaicin-pretreated with SB-334867-A (80 nM), groups of rat. However, the effect of capsaicin on COX-2 and BDNF expressions was reversed by a Vc microinjection of orexin-A (100 pM). Conclusions: Overall, the present data reveals that orexin-A can attenuate capsaicin-induced trigeminal pain through the modulation of pain effects on COX-2 and BDNF expressions in the Vc of rats.
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收藏
页码：174 / 182
页数：9
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