Interleukin-10 gene promoter polymorphism in English and Polish healthy controls.: Polymerase chain reaction haplotyping using 3′ mismatches in forward and reverse primers

被引：38
作者：
Koss, K
Fanning, GC
Welsh, KI
Jewell, DP
机构：
[1] Radcliffe Infirm, Gastroenterol Unit, Oxford OX2 6HE, England
[2] Med Univ Gdansk, Dept Gastroenterol, Gdansk, Poland
[3] Churchill Hosp, Nuffield Dept Surg, Oxford OX3 7LJ, England
来源：
GENES AND IMMUNITY | 2000年 / 1卷 / 05期
关键词：
genotyping; haplotyping; interleukin-10; polymorphism; polymerase chain reaction;
D O I：
10.1038/sj.gene.6363669
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A polymerase chain reaction with sequence-specific primers (PCR-SSP) system using primers with mismatches at the 3' ends was developed to determine polymorphisms in IL-10 promoter region. Three previously described biallelic polymorphisms in IL-10 were linked in a 12 reaction PCR-SSP system and the method used to provide genotype data on 233 UK and 166 Polish controls. There are eight possible polymorphic combinations in IL-10 promoter gene but only three were observed in both control groups. Population frequencies of IL-10 genotypes show, in contrast to HLA, that UK and Polish frequencies are remarkably similar.
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页码：321 / 324
页数：4
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