Looking into live cells with in-cell NMR spectroscopy

被引：104

作者：

Selenko, Philipp ^{[1
]}

Wagner, Gerhard ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2007年 / 158卷 / 02期

关键词：

high-resolution NMR spectroscopy; in-cell NMR spectroscopy; protein transduction; Xenopus laevis oocytes;

D O I：

10.1016/j.jsb.2007.04.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In-cell NMR spectroscopy has gained recent popularity since it provides means to analyze the conformational and functional properties of proteins inside living cells and at atomic resolution. High-resolution in-cell NMR spectroscopy was originally established in bacterial cells and based on a rationale that relies on protein over-expression and sample analysis within the same cellular environment. Here, we review in-cell NMR approaches in Xenopus laevis oocytes and evaluate potential future applications in other eukaryotic cell types. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：244 / 253

页数：10

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