Describing the structure and assembly of protein filaments by EPR spectroscopy of spin-labeled side chains

被引：7
作者：
Budamagunta, Madbu S.
Hess, John F.
Fitzgerald, Paul G.
Voss, John C. [1 ]
机构：
[1] Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USA
[2] Univ Calif Davis, Sch Med, Dept Cell Biol & Human Anat, Davis, CA 95616 USA
来源：
CELL BIOCHEMISTRY AND BIOPHYSICS | 2007年 / 48卷 / 01期
关键词：
intermediate filaments; vimentin; EPR (ESR) spectroscopy; site-directed spin labeling;
D O I：
10.1007/s12013-007-0035-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this review we summarize our approach to the study of Intermediate Filament (IF) structure and assembly by electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels. Using vimentin, a homopolymeric type III IF protein, we demonstrate that this approach serves as a general paradigm for studying protein filament structure and assembly. These strategies will be useful in exploring the structure and assembly properties of other filamentous or aggregation-prone systems.
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收藏
页码：45 / 53
页数：9
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