Determination of free and protein amino acid content in microalgae by HPLC-DAD with pre-column derivatization and pressure hydrolysis

被引：41

作者：

Araya, Michael ^{[1
]}

Garcia, Samantha ^{[1
]}

Rengel, Jose ^{[2
]}

Pizarro, Sebastian ^{[3
]}

Alvarez, Gonzalo ^{[1
,2
]}

机构：

[1] Univ Catolica Norte, Fac Ciencias Mar, Ctr Invest & Desarrollo Tecnol Algas CIDTA, Coquimbo, Chile

[2] Univ Catolica Norte, Fac Ciencias Mar, Dept Acuicultura, Coquimbo, Chile

[3] Univ La Serena, Fac Ciencias, Dept Quim, La Serena, Chile

来源：

MARINE CHEMISTRY | 2021年 / 234卷

关键词：

Microalgae; Amino acids; OPA; HPLC-DAD; INTRACELLULAR NITROGEN; MARINE MICROALGAE; CONVERSION; PHARMACEUTICALS; EXTRACTION; PRODUCTS; ALGAE;

D O I：

10.1016/j.marchem.2021.103999

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The content of free and hydrolyzed primary amino acids was determined for five species of microalgae: Haematococcus pluvialis, Skeletonema costatum, Arthrospira sp., Acutodesmus acuminatus, and Botryococcus braunii. The amino acid profile was determined using an automated HPLC-DAD reverse phase pre-column analysis method, which is described in detail. Acid hydrolysis using 6 M HCl and autoclaving at 120 degrees C for 2 h was validated using casein of bovine origin as standard. Nitrogen to protein conversion factors were calculated for each microalgae. Haematococcus pluvialis contains the least amount of free amino acids (38.8 +/- 0.15 mg AA/100 g DW), while Arthrospira sp. shows the most enriched free amino acid profile (316.1 +/- 0,11 mg AA/100 g DW). Skeletonema costatum shows the least amount of amino acids after hydrolysis with 5.09 +/- 0.28 g AA/100 g DW while Arthrospira sp. achieves 27.76 +/- 0.19 g AA/100 g DW. The total protein content and its relationship with the detected amino acid content are discussed.

引用

页数：7

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