The Serum-Resistant Transfection Evaluation and Long-Term Stability of Gene Delivery Dry Powder Based on Mesoporous Silica Nanoparticles and Polyethyleneimine by Freezing-Drying

被引：13

作者：

Zhang, Xiaoxu ^{[1
,2
]}

Zhang, Jianpan ^{[1
,2
]}

Quan, Guilan ^{[1
,2
]}

Yang, Pianpian ^{[1
,2
]}

Pan, Xin ^{[1
,2
]}

Wu, Chuanbin ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, 132 East Circle & Univ Town, Guangzhou 510006, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Res & Dev Ctr Pharmaceut Engn, Guangzhou, Guangdong, Peoples R China

来源：

AAPS PHARMSCITECH | 2017年 / 18卷 / 05期

基金：

中国国家自然科学基金;

关键词：

freeze-drying; gene; mesoporous silica nanoparticles; polyethyleneimine; transfection; SIRNA; THERAPY; DESIGN; POLYAMIDOAMINE; STABILIZATION; DOXORUBICIN; EFFICACY; SYSTEM;

D O I：

10.1208/s12249-016-0617-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Mesoporous silica nanoparticles (MSNs) with large surface area, tunable pore size, and low toxicity can act as suitable vehicles for drug and gene delivery. An MSN/DNA/PEI complex delivery system was prepared by using MSNs to hold plasmid DNA coated with polyethyleneimine (PEI), and the dry powder formulation was produced by freeze-drying with trehalose as lyoprotectant. The MSN/DNA/PEI complexes successfully enhanced the gene expression with about 1.5-fold higher efficiency as compared with the control, and even better effects and lower toxicity were achieved at lower content of PEI. Also, this gene delivery system showed nearly sixfold higher efficiency in the serum-containing condition than the control, so further application of these vehicles in vivo is highly appreciated. Besides, the trehalose containing lyophilized formulation could hold the availability for at least 4 months of storing at room temperature, presenting the potential for industrial production and transportation of gene therapy.

引用

页码：1536 / 1543

页数：8

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