Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues

被引：4
作者：
Kim, Duyeol [1 ]
Ha, Yooncheol [1 ]
Oh, Yeonsu [1 ]
Han, Kiwon [1 ]
Noh, Seung Ho [2 ]
Kim, Cheolwoo [3 ]
Kim, Sung-Hoon [3 ]
Chae, Chanhee [1 ]
机构：
[1] Seoul Natl Univ, Coll Vet Med, Dept Vet Pathol, Seoul 151742, South Korea
[2] Hanyang Univ, Coll Engn, Dept Biotechnol, Seoul 133791, South Korea
[3] Kyung Hee Univ, Coll Oriental Med, Seoul, South Korea
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2010年 / 22卷 / 02期
关键词：
Formalin-fixed tissue; genotype; in situ hybridization; Porcine circovirus-2; MULTISYSTEMIC WASTING SYNDROME; PORCINE CIRCOVIRUS; DEFINITION; TYPE-2; PIGS;
D O I：
10.1177/104063871002200209
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of the current study was to develop a nonradioactive in situ hybridization assay that can differentiate between genotypes 221 and 2b of Porcine circovirus-2 (PCV-2) in formalin-fixed, paraffin-embedded lymph node tissues from pigs with postweaning multisystemic wasting syndrome. Two different digoxigenin-labeled oligonucleotide probes were designed from the PCV-2 open reading frame 2 sequences. The PCV-2a-specific probe did not hybridize with formalin-fixed, paraffin-embedded lymph nodes from naturally PCV-2b-infected pigs and vice versa. Both PCV-2a-specific and PCV-2b-specific probes gave consistent negative signals in lymph nodes from naturally PCV-1-infected pigs. The in situ hybridization assay described in the present study represents a diagnostic tool that can differentiate between the 2 genotypes of PCV-2.
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收藏
页码：231 / 233
页数：3
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