SYNTHESIS OF 2-(3,4-DIHYDRO-3-OXO-2H-[1,2,4]TRIAZINO[4,3-c]QUINAZOLIN-4-YL)ACETIC ACID DERIVATIVES AS POTENTIAL ANTIOXIDANTS IN NITROSATIVE STRESS MODEL

The esters (3a-f) and amides (4a-c) of 2-(3,4-dihydro-3-oxo-2H-[1,2,4]triazino[4,3-c]-quinazolin-4-yl)acetic acid were synthesized by prior esterification or aminolysis of the acid 2. The structure of the synthesized compounds was confirmed by spectroscopic investigations. Almost all substances, which have been tested in vitro for the antioxidant activity in nitrosative stress, have shown significant antioxidant activity in comparison with N-acetylcystein (N-ACC). Compound 3f revealed the best antioxidant properties in a nitrosative stress model. Few weeks after the implementation of the new legislation, it seems that its purpose has at least partially been achieved; a number of "dreams shops" closed or changed their field of activity. However, it is possible that some of the consumers of such products, which have rather moderate risks, will replace them with drugs associated with higher risks, acquired from the black market. Whether the general outcome would be positive or negative will remain to be seen.