Effects of Embryonic Neural Stem Cell Transplantation on DNA Damage in the Brain and Spinal Cord Following Spinal Cord Injury

被引：2

作者：

Dagci, T. ^{[2
]}

Konyalioglu, S. ^{[3
]}

Keser, A. ^{[2
]}

Kayalioglu, G. ^{[1
]}

机构：

[1] Ege Univ, Dept Anat, Sch Med, Izmir, Turkey

[2] Ege Univ, Dept Physiol, Sch Med, Izmir, Turkey

[3] Ege Univ, Dept Biochem, Fac Pharm, Izmir, Turkey

来源：

NEUROPHYSIOLOGY | 2009年 / 41卷 / 06期

关键词：

spinal cord injury; stem cells; DNA damage; Comet assay; MITOCHONDRIAL CYTOCHROME-C; NITRIC-OXIDE SYNTHASE; OXIDATIVE STRESS; SUPEROXIDE-DISMUTASE; NAD(P)H OXIDASE; RATS; ACTIVATION; EXPRESSION; APOPTOSIS; CASPASE-3;

D O I：

10.1007/s11062-010-9120-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Embryonic neural stem cell (ENSC) transplantation is used experimentally for the improvement of spinal cord repair following spinal cord injury (SCI). However, the effects of such intervention on oxidative stress and cell death remain unknown. We used in vivo Comet assay in the acute and chronic SCI groups compared with the SCI+ENSC transplantation groups of experimental rats in order to evaluate DNA damage in the spinal cord. Chronic SCI resulted in the generation of oxidative DNA damage in the spinal cord brain and kidneys, as indicated by high Comet assay parameters, including the percentage of DNA in the tail (T%, or TD), tail moment (TM), and tail length (TL). The DNA damage levels significantly decreased after ENSC transplantation in the spinal cords of acute and chronic SCI groups within the lesion site and rostrally and caudally to the injury, and in the brains and kidneys of the chronic SCI group. Thus, ENSC transplantation is found to be an effective tool for limitation of DNA damage following spinal cord injury.

引用

页码：409 / 415

页数：7

共 36 条

[1] Impaired mitochondrial function, oxidative stress and altered antioxidant enzyme activities following traumatic spinal cord injury [J].