Determination of Antidepressants Using Monoamine Oxidase Amperometric Biosensors Based on Screen-Printed Graphite Electrodes Modified with Multi-Walled Carbon Nanotubes

被引：7

作者：

Medyantseva, E. P. ^{[1
]}

Brusnitsyn, D. V. ^{[1
]}

Varlamova, R. M. ^{[1
]}

Baibatarova, M. A. ^{[1
]}

Budnikov, G. K. ^{[1
]}

Fattakhova, A. N. ^{[2
]}

机构：

[1] Kazan Fed Univ, AM Butlerov Chem Inst, Kazan 420008, Tatarstan, Russia

[2] Kazan Fed Univ, Inst Fundamental Med & Biol, Kazan 420008, Tatarstan, Russia

来源：

PHARMACEUTICAL CHEMISTRY JOURNAL | 2014年 / 48卷 / 07期

基金：

俄罗斯基础研究基金会;

关键词：

amperometric biosensor; carbon nanotubes; antidepressants; afobazole; imipramine;

D O I：

10.1007/s11094-014-1135-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Novel amperometric biosensors based on screen-printed graphite electrodes modified with multi-walled carbon nanotubes (MWCNTs) dispersed in various solvents (chitosan, DMF) and immobilized monoamine oxidase (MAO) were developed for the determination of the antidepressants imipramine and afobazole. It was found that afobazole and imipramine inhibited MAO, which allowed these pharmaceuticals to be determined within the concentration range from 0.1 mM to 1 nM by recording the H2O2 oxidation wave when dopamine and epinephrine were used as MAO substrates. The best analytical performance was observed for MAO biosensors modified by MWCNTs in chitosan solution, for which the lower detection limit was 3 - 9 nM. The main active ingredients in melipramine and afobazole preparations were quantified with an RSD of 0.08 using the MAO-based amperometric biosensors.

引用

页码：478 / 482

页数：5

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