Characterization of Chicken MMP13 Expression and Genetic Effect on Egg Production Traits of Its Promoter Polymorphisms

被引:18
作者
Yuan, Zhenjie [1 ]
Chen, Yuxia [2 ]
Chen, Qiuyue [2 ]
Guo, Miao [2 ]
Kang, Li [2 ]
Zhu, Guiyu [3 ]
Jiang, Yunliang [2 ]
机构
[1] Shandong Agr Univ, Coll Agron, Dept Genet, Tai An 271018, Shandong, Peoples R China
[2] Shandong Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China
[3] Taishan Univ, Dept Biol Sci & Technol, Tai An 271000, Shandong, Peoples R China
来源
G3-GENES GENOMES GENETICS | 2016年 / 6卷 / 05期
基金
美国国家科学基金会;
关键词
chicken; MMP13; follicle; polymorphism; age at first laying; TRANSCRIPTION FACTOR KLF7; MATRIX-METALLOPROTEINASE SYSTEM; MESSENGER-RIBONUCLEIC-ACID; EXTRACELLULAR-MATRIX; RAT OVARY; COLLAGENASE-3; MMP-13; GALLUS-DOMESTICUS; OVULATORY PROCESS; INHIBITORS; DIFFERENTIATION;
D O I
10.1534/g3.116.027755
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Extracelluar matrix undergoes constant remodeling, cell-cell, and cell-matrix interactions during chicken ovarian follicle growth, which is coordinated by matrix metalloproteinases (MMPs), and their associated endogenous inhibitors (TIMPs). Transcriptome analysis revealed upregulation of MMP13 in sexually mature chicken ovaries. In this study, we found that the expression of MMP13 in chicken ovary was stably elevated from 60 d to 159 d, and was significantly higher at 159 d than at the other three developmental stages (P < 0.05). The expression of MMP13 mRNA increased from SW (small white follicles) to F5 (fifth largest follicles), then decreased to F1 (first largest follicles), and dramatically increased again in POF1 (newly postovulatory follicles) follicles (P < 0.05). The MMP13 protein was localized in stroma cells and primordial follicles of sexually immature chicken ovaries, in the theca cell layers of all sized follicles of sexually mature chicken ovaries. Furthermore, we identified a positive element (positions -1863 to -1036) controlling chicken MMP13 transcription, and, in this region, six single nucleotide polymorphisms were found and genotyped in chicken populations. In the White Recessive Rock population, hens with A(-1356)-C-1079/A(-1356)-C-1079 genotype had earlier "age at first laying" than those with G(-1356)-T-1079/G(-1356)-T-1079 genotype (P < 0.05), and exhibited significantly lower transcriptional activity (P < 0.01). Collectively, chicken MMP13 plays an important role in ovarian follicle growth and regression, and polymorphisms in its promoter region could be used as molecular markers for improving the trait "age at first laying" in chicken breeding.
引用
收藏
页码:1305 / 1312
页数:8
相关论文
共 36 条
  • [1] Gonadotropin surge-induced differential upregulation of collagenase-1 (MMP-1) and collagenase-3 (MMP-13) mRNA and protein in bovine preovulatory follicles
    Bakke, LJ
    Li, QL
    Cassar, CA
    Dow, MPD
    Pursley, JR
    Smith, GW
    [J]. BIOLOGY OF REPRODUCTION, 2004, 71 (02) : 605 - 612
  • [2] Expression of collagenase-3 in the rat ovary during the ovulatory process
    Balbin, M
    Fueyo, A
    Lopez, JM
    DiezItza, I
    Velasco, G
    LopezOtin, C
    [J]. JOURNAL OF ENDOCRINOLOGY, 1996, 149 (03) : 405 - 415
  • [3] Transcription factor KLF7 regulates differentiation of neuroectodermal and mesodermal cell lineages
    Caiazzo, Massimiliano
    Colucci-D'Amato, Luca
    Esposito, Maria T.
    Parisi, Silvia
    Stifani, Stefano
    Ramirez, Francesco
    di Porzio, Umberto
    [J]. EXPERIMENTAL CELL RESEARCH, 2010, 316 (14) : 2365 - 2376
  • [4] Collagenase and gelatinase messenger ribonucleic acid expression and activity during follicular development in the rat ovary
    Cooke, RG
    Nothnick, WB
    Komar, C
    Burns, P
    Curry, TE
    [J]. BIOLOGY OF REPRODUCTION, 1999, 61 (05) : 1309 - 1316
  • [5] Cyclic changes in the matrix metalloproteinase system in the ovary and uterus
    Curry, TE
    Osteen, KG
    [J]. BIOLOGY OF REPRODUCTION, 2001, 64 (05) : 1285 - 1296
  • [6] The matrix metalloproteinase system: Changes, regulation, and impact throughout the ovarian and uterine reproductive cycle
    Curry, TE
    Osteen, KG
    [J]. ENDOCRINE REVIEWS, 2003, 24 (04) : 428 - 465
  • [7] Impact of extracellular matrix remodeling on ovulation and the folliculo-luteal transition
    Curry, Thomas E., Jr.
    Smith, Michael F.
    [J]. SEMINARS IN REPRODUCTIVE MEDICINE, 2006, 24 (04) : 228 - 241
  • [8] Cellular turnover and extracellular matrix remodeling in female reproductive tissues: functions of metalloproteinases and their inhibitors
    Fata, JE
    Ho, ATV
    Leco, KJ
    Moorehead, RA
    Khokha, R
    [J]. CELLULAR AND MOLECULAR LIFE SCIENCES, 2000, 57 (01) : 77 - 95
  • [9] FREIJE JMP, 1994, J BIOL CHEM, V269, P16766
  • [10] GILBERT AB, 1983, J REPROD FERTIL, V69, P221, DOI 10.1530/jrf.0.0690221