Microsomal glutathione transferase (mGST) is a membrane bound glutathione transferase in multifunctional detoxification isoenzymes family and also plays crucial roles in innate immunity. In the present study, a novel microsomal GST homology was identified from Apostichopus japonicus (designated as AjmGST1) by RACE approaches. The full-length cDNA of AjmGST1 was of 1296 bp encoded a protein of 169 amino acids residues. Multiple sequence alignment and phylogenetic analysis together supported that AjmGST1 belonged to a new member in invertebrates mGST family. Spatial expression analysis revealed that AjmGSTl was ubiquitously expressed in all examined tissues with the larger magnitude in tentacle. Time-course expression of AjmGST1 mRNA in coelomocytes was up-regulated after Vibrio splendidus challenge from 6 h until 72 h with the peak expression in 24 h, compared with that in the control group. Similarly, the induced expression of AjmGSTI expression was also detected in lipopolysaccharide (LPS) exposed primary coelomocytes. The purified recombinant protein of AjmGST1 showed high activity with GST substrate at pH of 7.0 and temperature of 35 degrees C. Meantime, the recombinant AjmGST1 depressed H2O2-induced MDA production both in vivo and in vitro. All of these results indicated that AjmGST1 was an important regulator in elimination of lipid peroxidation under immune response. (C) 2017 Elsevier Ltd. All rights reserved.
机构:
Karolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
Johansson, Katarina
Jarvliden, Julia
论文数: 0引用数: 0
h-index: 0
机构:
Karolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
Jarvliden, Julia
Gogvadze, Vladimir
论文数: 0引用数: 0
h-index: 0
机构:
Karolinska Inst, Div Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
Gogvadze, Vladimir
Morgenstern, Ralf
论文数: 0引用数: 0
h-index: 0
机构:
Karolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Li, Chenghua
Feng, Weida
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Feng, Weida
Qiu, Lihua
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Fishery Sci, S China Sea Fishery Res Inst, Guangzhou 510301, Guangdong, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Qiu, Lihua
Xia, Changge
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Xia, Changge
Su, Xiurong
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Su, Xiurong
Jin, Chunhua
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Jin, Chunhua
Zhou, Tingting
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Zhou, Tingting
Zeng, Yuan
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Zeng, Yuan
Li, Taiwu
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Ningbo City Coll Vocat Technol, Ningbo 315100, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
机构:
Karolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
Johansson, Katarina
Jarvliden, Julia
论文数: 0引用数: 0
h-index: 0
机构:
Karolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
Jarvliden, Julia
Gogvadze, Vladimir
论文数: 0引用数: 0
h-index: 0
机构:
Karolinska Inst, Div Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
Gogvadze, Vladimir
Morgenstern, Ralf
论文数: 0引用数: 0
h-index: 0
机构:
Karolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, SwedenKarolinska Inst, Div Biochem Toxicol, Inst Environm Med, SE-17177 Stockholm, Sweden
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Li, Chenghua
Feng, Weida
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Feng, Weida
Qiu, Lihua
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Fishery Sci, S China Sea Fishery Res Inst, Guangzhou 510301, Guangdong, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Qiu, Lihua
Xia, Changge
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Xia, Changge
Su, Xiurong
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Su, Xiurong
Jin, Chunhua
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Jin, Chunhua
Zhou, Tingting
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Zhou, Tingting
Zeng, Yuan
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Zeng, Yuan
Li, Taiwu
论文数: 0引用数: 0
h-index: 0
机构:
Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
Ningbo City Coll Vocat Technol, Ningbo 315100, Zhejiang, Peoples R ChinaNingbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China