Intracellular Delivery of a Membrane-Impermeable Enzyme in Active Form Using Functionalized Gold Nanoparticles

被引：162

作者：

Ghosh, Partha ^{[1
]}

Yang, Xiaochao ^{[1
,2
,3
]}

Arvizo, Rochelle ^{[1
]}

Zhu, Zheng-Jiang ^{[1
]}

Agasti, Sarit S. ^{[1
]}

Mo, Zhihong ^{[2
,3
]}

Rotello, Vincent M. ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

[2] Chongqing Univ, Coll Bioengn, Chongqing 400044, Peoples R China

[3] Chongqing Univ, Microsyst Res Ctr, Chongqing 400044, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 08期

关键词：

ARGININE-RICH PEPTIDES; NONVIRAL GENE DELIVERY; PROTEIN DELIVERY; SURFACE RECOGNITION; MAMMALIAN-CELLS; DRUG-DELIVERY; RECEPTORS; POLYMERS; INTERNALIZATION; TRANSLOCATION;

D O I：

10.1021/ja907887z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.

引用

页码：2642 / 2645

页数：4

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