Fucoxanthin Attenuates Free Fatty Acid-Induced Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism/Oxidative Stress/Inflammation via the AMPK/Nrf2/TLR4 Signaling Pathway

被引:27
|
作者
Ye, Jiena [1 ]
Zheng, Jiawen [1 ]
Tian, Xiaoxiao [1 ]
Xu, Baogui [1 ]
Yuan, Falei [1 ]
Wang, Bin [1 ]
Yang, Zuisu [1 ]
Huang, Fangfang [1 ]
机构
[1] Zhejiang Ocean Univ, Zhejiang Prov Engn Technol Res Ctr Marine Biomed, Sch Food & Pharm, Zhoushan 316022, Peoples R China
基金
中国国家自然科学基金;
关键词
NAFLD; fucoxanthin; adenosine monophosphate-activated protein kinase (AMPK); nuclear factor erythroid-2-related factor 2-mediated (Nrf2); Toll-like receptor 4-mediated (TLR4); OXIDATIVE STRESS; CELL-DEATH; ACCUMULATION; INFLAMMATION; INJURY; STEATOHEPATITIS; HEPATOCYTES; ACTIVATION; TRENDS; MODEL;
D O I
10.3390/md20040225
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Fucoxanthin, a xanthophyll carotenoid abundant in brown algae, is reported to have several biological functions, such as antioxidant, anti-inflammatory, and anti-tumor activities, in mice. We investigated the effects and mechanisms of fucoxanthin in the mixture oleate/palmitate = 2/1(FFA)-induced nonalcoholic fatty liver disease (NAFLD) cell model in this study. The results showed that the content of superoxide dismutase in the FFA group was 9.8 +/- 1.0 U/mgprot, while that in the fucoxanthin high-dose (H-Fx) group (2 mu g/mL) increased to 22.9 +/- 0.6 U/mgprot. The content of interleukin-1 beta in the FFA group was 89.3 +/- 3.6 ng/mL, while that in the H-Fx group was reduced to 53.8 +/- 2.8 ng/mL. The above results indicate that fucoxanthin could alleviate the FFA-induced oxidative stress and inflammatory levels in the liver cells. Oil red-O staining revealed visible protrusions and a significant decrease in the number of lipid droplets in the cytoplasm of cells in the fucoxanthin group. These findings on the mechanisms of action suggest that fucoxanthin can repair FFA-induced NAFLD via the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway and nuclear factor erythroid-2-related factor 2-mediated (Nrf2) signaling pathway, as well as by downregulating the expression of the Toll-like receptor 4-mediated (TLR4) signaling pathway. Fucoxanthin exhibited alleviating effects in the FFA-induced NAFLD model and could be explored as a potential anti-NAFLD substance.
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页数:14
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