Specific Imaging of Intracellular Lipid Droplets Using a Benzothiadiazole Derivative with Solvatochromic Properties

被引：53

作者：

Appelqvist, Hanna ^{[1
]}

Stranius, Kati ^{[2
]}

Borjesson, Karl ^{[2
]}

Nilsson, K. Peter. R. ^{[1
]}

Dyrager, Christine ^{[1
]}

机构：

[1] Linkoping Univ, Dept Phys Chem & Biol, S-58183 Linkoping, Sweden

[2] Univ Gothenburg, Dept Chem & Mol Biol, S-41296 Gothenburg, Sweden

来源：

BIOCONJUGATE CHEMISTRY | 2017年 / 28卷 / 05期

基金：

瑞典研究理事会;

关键词：

NILE RED; FLUORESCENT-PROBE; CELL BIOLOGY; CANCER; 2,1,3-BENZOTHIADIAZOLE; FLUOROPHORES; AGGREGATION; SURVIVAL; BODIPY; FUSION;

D O I：

10.1021/acs.bioconjchem.7b00048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Altered lipid metabolism and extensive lipid storage in cells have been associated with various medical disorders, including cancer. The development of fluorescent probes that specifically accumulate in lipid deposits is therefore of great interest in order to study pathological processes that are linked to dysregulated lipogenesis. In the present study, we present a small fluorescent benzothiadiazole dye that specifically stains lipid droplets in living and fixated cells. The photophysical characterization of the probe revealed strong solvatochromic behavior, large Stokes shifts, and high fluorescent quantum yields in hydrophobic solvents. In addition, the fluorophore exhibits a nontoxic profile and a high signal-to-noise ratio in cells (i.e., lipid droplets vs cytosol), which make it an excellent candidate for studying lipid biology using confocal fluorescent microscopy.

引用

页码：1363 / 1370

页数：8

共 53 条

[1]

[Anonymous], SIGNAL TRANSDUCTION

[2]

[Anonymous], FLUORESCENT DETECTIO

[3] Lipid droplets: A dynamic organelle moves into focus [J].

Beller, Mathias ;

Thiel, Katharina ;

Thul, Peter J. ;

Jaeckle, Herbert .

FEBS LETTERS, 2010, 584 (11) :2176-2182

[4] Lipid metabolic reprogramming in cancer cells [J].

Beloribi-Djefaflia, S. ;

Vasseur, S. ;

Guillaumond, F. .

ONCOGENESIS, 2016, 5 :e189-e189

[5] Weighing in on Adipocyte Precursors [J].

Berry, Ryan ;

Jeffery, Elise ;

Rodeheffer, Matthew S. .

CELL METABOLISM, 2014, 19 (01) :8-20

[6] Lipid droplets fusion in adipocyte differentiated 3T3-L1 cells: A Monte Carlo simulation [J].

Boschi, Federico ;

Rizzatti, Vanni ;

Zamboni, Mauro ;

Sbarbati, Andrea .

EXPERIMENTAL CELL RESEARCH, 2014, 321 (02) :201-208

[7] Lipid droplets in inflammation and cancer [J].

Bozza, Patricia T. ;

Viola, Joao P. B. .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 2010, 82 (4-6) :243-250

[8] Fundamental aspects of property tuning in push-pull molecules [J].

Bures, Filip .

RSC ADVANCES, 2014, 4 (102) :58826-58851

[9] Designed Benzothiadiazole Fluorophores for Selective Mitochondrial Imaging and Dynamics [J].

Carvalho, Pedro H. P. R. ;

Correa, Jose R. ;

Guido, Bruna C. ;

Gatto, Claudia C. ;

De Oliveira, Heibbe C. B. ;

Soares, Thereza A. ;

Neto, Brenno A. D. .

CHEMISTRY-A EUROPEAN JOURNAL, 2014, 20 (47) :15360-15374

[10] Design, synthesis and application of fluorescent 2,1,3-benzothiadiazole-triazole-linked biologically active lapachone derivatives [J].

da Cruz, Eduardo H. G. ;

Carvalho, Pedro H. P. R. ;

Correa, Jose R. ;

Silva, Diego A. C. ;

Diogo, Emilay B. T. ;

de Souza Filho, Jose D. ;

Cavalcanti, Bruno C. ;

Pessoa, Claudia ;

de Oliveira, Heibbe C. B. ;

Guido, Bruna C. ;

da Silva Filho, Demetrio A. ;

Neto, Brenno A. D. ;

da Silva Junior, Eufranio N. .

NEW JOURNAL OF CHEMISTRY, 2014, 38 (06) :2569-2580

← 1 2 3 4 5 6 →