Spectroscopic studies on the interaction between silicotungstic acid and bovine serum albumin

被引：154

作者：

Wang, Yan-Qing ^{[1
]}

Zhang, Hong-Mei ^{[1
]}

Zhang, Gen-Cheng ^{[1
]}

Tao, Wei-Hua ^{[1
]}

Fei, Zheng-Hao ^{[1
]}

Liu, Zong-Tang ^{[1
]}

机构：

[1] Yancheng Normal Coll, Inst Appl Chem & Environm ENgn, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224002, Jiangsu, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 43卷 / 05期

关键词：

silicotungstic acid; bovine serum albumin; UV/vis spectroscopy; fluorescence spectroscopy; thermodynamic parameters;

D O I：

10.1016/j.jpba.2007.01.001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The interaction between silicotungstic acid and bovine serum albumin (BSA) was investigated using fluorescence and UV/vis. The experimental results showed that the fluorescence quenching of BSA by silicotungstic acid is a result of the formation of SiW-BSA complex; static quenching and non-radiative energy transferring were confirmed to result in the fluorescence quenching. The binding site number n, apparent binding constant K-A and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BSA was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Hydrophobic interaction force plays a major role in stabilizing the complex. The effect of silicotungstic acid on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1869 / 1875

页数：7

共 43 条

[11] HU YJ, 2005, J PHARM BIOMED ANAL, V69, P740
[12] Interaction of ochratoxin A with human serum albumin. Preferential binding of the dianion and pH effects
Il'ichev, YV
Perry, JL
Simon, JD
[J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (02) : 452 - 459
[13] JASMIN C, 1973, BIOMEDICINE, V18, P319
[14] Spectroscopic studies on the interaction of cinnamic acid and its hydroxyl derivatives with human serum albumin
Jiang, M
Xie, MX
Zheng, D
Liu, Y
Li, XY
Chen, X
[J]. JOURNAL OF MOLECULAR STRUCTURE, 2004, 692 (1-3) : 71 - 80
[15] Covalent binding of the flavonoid quercetin to human serum albumin
Kaldas, MI
Walle, UK
Van der Woude, H
McMillan, JM
Walle, T
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (10) : 4194 - 4197
[16] Fluorescence studies on PAMAM dendrimers interactions with bovine serum albumin
Klajnert, B
Bryszewska, M
[J]. BIOELECTROCHEMISTRY, 2002, 55 (1-2) : 33 - 35
[17] PHYSICOCHEMICAL ASPECTS OF DRUG-PROTEIN INTERACTIONS - GENERAL PERSPECTIVE
KLOTZ, IM
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1973, 226 (NOV26) : 18 - 35
[18] KRAGHHANSEN U, 1981, PHARMACOL REV, V33, P17
[19] Ladokhin A. S., 2000, ENCY ANAL CHEM, P5762
[20] Effect of metal ions on the interaction between bovine serum albumin and berberine chloride extracted from a traditional Chinese Herb coptis chinensis franch
Liu, XF
Xia, YM
Fang, Y
[J]. JOURNAL OF INORGANIC BIOCHEMISTRY, 2005, 99 (07) : 1449 - 1457

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