First molecular investigation of Cryptosporidium spp. in young calves in Algeria

被引:24
作者
Benhouda, Djahida [1 ]
Hakem, Ahcene [1 ]
Sannella, Anna Rosa [2 ]
Benhouda, Afaf [3 ]
Caccio, Simone M. [2 ]
机构
[1] Univ Ziane Achour, Fac SNV, Lab Explorat & Valorizat Stepp Ecosyst, Djelfa 17000, Algeria
[2] Ist Super Sanita, Dept Infect Dis, I-00161 Rome, Italy
[3] Univ Batna 2, Fac Biol Sci, Dept Living Organisms, Biotechnol Lab Bioact Mol & Cellular Physiopathol, Batna 05000, Algeria
关键词
Cryptosporidium; Cryptosporidiosis; Molecular characterization; Cattle; Algeria; DAIRY CALVES; PARVUM; SUBTYPES; HUMANS; CATTLE; EPIDEMIOLOGY; GENOTYPES; ETIOLOGY; DISEASE; BURDEN;
D O I
10.1051/parasite/2017014
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
To date, no information is available on the prevalence and genetic identity of Cryptosporidium spp. in cattle in Algeria. In this study, 17 dairy farms in the province of Batna, located in the northeast of the country, were visited to collect 132 fecal samples from young calves (< 8 weeks old). Samples were examined microscopically using the modified Ziehl-Neelsen acid-fast staining method, and at least one sample per farm was submitted for molecular analysis. Amplification of a fragment of the small subunit ribosomal RNA gene was positive for 24 of the 61 samples (40%), and sequence analysis identified three species, namely Cryptosporidium bovis (n = 14), C. ryanae (n = 6), and C. parvum (n = 4). The C. parvum IIaA13G2R1 subtype, an uncommon zoonotic subtype, was identified in two isolates from a single farm by sequencing a fragment of the GP60 gene. This is the first report about genotyping and subtyping of Cryptosporidium in calves in Algeria.
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