Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp

被引:30
作者
Li, Taotao [1 ,2 ,3 ]
Yun, Ze [1 ,2 ,3 ]
Wu, Qixian [1 ,2 ,3 ,4 ]
Qu, Hongxia [1 ,2 ,3 ]
Duan, Xuewu [1 ,2 ,3 ]
Jiang, Yueming [1 ,2 ,3 ]
机构
[1] Chinese Acad Sci, Key Lab Post Harvest Handling Fruits, Minist Agr, Guangzhou 510650, Guangdong, Peoples R China
[2] Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, Guangzhou 510650, Guangdong, Peoples R China
[3] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
[4] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
基金
中国国家自然科学基金;
关键词
fruit; softening; auxin; proteomic; transcriptomic; signal transduction; GENE-EXPRESSION; FRUIT; SENESCENCE; ETHYLENE; PROTEINS; GENOME;
D O I
10.3390/biom9100523
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The banana is one of the most important fruits in the world. Bananas undergo a rapid ripening process after harvest, resulting in a short shelf. In this study, the mechanism underlying pulp ripening of harvested bananas was investigated using integrated transcriptomic, proteomic, and metabolomic analysis. Ribonucleic acid sequencing (RNA-Seq) revealed that a great number of genes related to transcriptional regulation, signal transduction, cell wall modification, and secondary metabolism were up-regulated during pulp ripening. At the protein level, 84 proteins were differentially expressed during pulp ripening, most of which were associated with energy metabolism, oxidation-reduction, cell wall metabolism, and starch degradation. According to partial least squares discriminant analysis, 33 proteins were identified as potential markers for separating different ripening stages of the fruit. In addition to ethylene's central role, auxin signal transduction might be involved in regulating pulp ripening. Moreover, secondary metabolism, energy metabolism, and the protein metabolic process also played an important role in pulp ripening. In all, this study provided a better understanding of pulp ripening of harvested bananas.
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页数:26
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