Immunity to Plasmodium falciparum malaria sporozoites by somatic transgene immunization

被引：30
作者：
Gerloni, M
Ballou, WR
Billetta, R
Zanetti, M
机构：
[1] UNIV CALIF SAN DIEGO, DEPT MED, LA JOLLA, CA 92093 USA
[2] UNIV CALIF SAN DIEGO, CTR CANC, LA JOLLA, CA 92093 USA
[3] WALTER REED ARMY INST RES, DEPT IMMUNOL, ROCKVILLE, MD USA
来源：
NATURE BIOTECHNOLOGY | 1997年 / 15卷 / 09期
关键词：
vaccine design; DNA immunization; malaria;
D O I：
10.1038/nbt0997-876
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Immunity against the human malaria parasite Plasmodium falciparum was induced using somatic transgene immunization, a method to effectively target B lymphocytes in vivo. A single inoculation of plasmid DNA containing an immunoglobulin heavy-chain gene coding in the complementarity-determining region 3 for three repeats of the sequence Asn-Ala-Asn-Pro (NANP), a B-cell epitope of Fl falciparum sporozoites, induced antibodies against NANP in all mice. A booster with an antibody antigenized with the NANP peptide, or challenge with P. falciparum sporozoites, demonstrated the establishment of immunologic memory, Immunity to a parasite antigen can be induced by exploiting mechanisms in which B lymphocytes are both the source of the immunogen as well as the effector mechanism of immunity. The results indicate that somatic transgene immunization is a potential approach for vaccination against foreign pathogens.
引用
收藏
页码：876 / 881
页数：6
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