The barley miR393 has multiple roles in regulation of seedling growth, stomatal density, and drought stress tolerance

被引：34

作者：

Yuan, Weiyi ^{[1
]}

Suo, Jingqi ^{[1
]}

Shi, Bo ^{[1
]}

Zhou, Chenlu ^{[1
]}

Bai, Bin ^{[1
]}

Bian, Hongwu ^{[1
]}

Zhu, Muyuan ^{[1
]}

Han, Ning ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, Inst Genet & Regenerat Biol, Key Lab Cell & Gene Engn Zhejiang Prov, Hangzhou 310058, Zhejiang, Peoples R China

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2019年 / 142卷

基金：

中国国家自然科学基金;

关键词：

miR393; Auxin response; Stomata; Drought tolerance; Barley; BOX PROTEIN TIR1; AUXIN-RESPONSE; ARABIDOPSIS; MODULE; CONTRIBUTES; GERMINATION; MICRORNA160; EXPRESSION; REPRESSION; RESISTANCE;

D O I：

10.1016/j.plaphy.2019.07.021

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

microRNA393 (miR393) and its target module have been implicated as comprising a conserved mechanism to regulate developmental processes and plant growth in response to environmental signals through the auxin signaling pathway. Our previous work identified miR393 and its two targets in barley. In this study, we further investigated the expression pattern of miR393 and its biological functions in seedling growth and drought tolerance. We showed that the miR393 overexpressing line (OE) exhibited increased stomatal density with decreased guard cell length, while the miR393 knockdown line (MIM) displayed the opposite phenotype, which might be due to the effects of miR393 on AUXIN RESPONSE FACTORS (ARFS) and three stomatal development related genes, such as EPIDERMAL PATTERNING FACTOR1 (EPFI), SPEECHLESS (SPCH), and MUTE. In addition, the MINI line conferred enhanced drought tolerance, with alleviated leaf chlorosis and lipid peroxidation after 22 days drought treatment. In contrast, the OE line was more sensitive to drought stress and accumulated more malondialdehyde and hydrogen peroxide than the wild type. Furthermore, polyethylene glycol (PEG) treatment induced abscisic acid (ABA) accumulation in leaves was suppressed in the OE line, indicating that miR393 might regulate drought stress response and tolerance through its interaction with ABA biosynthesis. Overall, these data suggest that miR393 might be a potential target for manipulation of stomatal density and improvement of drought tolerance in barley.

引用

页码：303 / 311

页数：9

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