Metal free, "click and click-click" conjugation of ribonucleosides and 2′-OMe oligoribonucleotides on the solid phase

被引：16
作者：
Singh, Ishwar [1 ]
Heaney, Frances [1 ]
机构：
[1] NUI Maynooth, Dept Chem, Maynooth, Kildare, Ireland
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2010年 / 8卷 / 02期
基金：
爱尔兰科学基金会;
关键词：
1,3-DIPOLAR CYCLOADDITION; TERMINAL ALKYNES; IN-VIVO; CHEMISTRY; DNA; DELIVERY; OLIGONUCLEOTIDES; NUCLEOSIDE; STRATEGIES; ANTISENSE;
D O I：
10.1039/b918463e
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A fast and practical metal free conjugation of ribonucleosides and 2'-OMe 4-mer oligoribonucleotides has been accomplished by a nitrile oxide alkyne click cycloaddition reaction on the solid-phase, the methodology is suited to modification at either, or both, the 3'- or the 5'-terminus of the oligoribonucleotide substrate.
引用
收藏
页码：451 / 456
页数：6
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