Production of transgenic Phalaenopsis plants by introducing glutathione S-transferase gene into protocorms at an early stage after germination

被引：5

作者：

Chin, D. P. ^{[1
]}

Mii, M. ^{[1
]}

Mishiba, K. I. ^{[2
]}

机构：

[1] Chiba Univ, Fac Hort, Lab Plant Cell Technol, 648 Matsudo, Matsudo, Chiba 2718510, Japan

[2] Osaka Prefecture Univ, Grad Sch Life & Environm, Plant Mol Biol Grp, 1-1 Gakuen Sakai, Osaka 5998531, Japan

来源：

PROCEEDINGS OF THE 22ND INTERNATIONAL EUCARPIA SYMPOSIUM SECTION ORNAMENTALS: BREEDING FOR BEAUTY, PT II | 2007年 / 743卷

关键词：

Agrobacterium tumefaciens; genetic transformation; orchid; over-expression; stress;

D O I：

10.17660/ActaHortic.2007.743.13

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Transgenic Phalaenopsis plants over-expressing glutathione S-transferase (GSI) gene were produced by Agrobacterium-mediated transformation method. Protocorms at an early stage after germination were inoculated with A. tumefaciens strain EHAIOI (pEKH35SI63P) that harbors GST, hygromycin phosphotransferase and neomycin phosphotransferase 11 genes. A total of 68 independent transgenic plants derived from 6325 mature seeds were obtained 6 months after infection with Agrobacterium. Regenerated plants were grown in greenhouse and all plants bloomed within 2 years. Five months after pollination, 13 seed pods were obtained from 6 plants showing suitable commercial characters. Germinated Phalaenopsis T! seedlings with GST gene were confirmed by PCR analysis. Northern blot analysis confirmed the expression of GST in hygromycin-resistant plantlets.

引用

页码：101 / +

页数：3

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