Genotyping and PCR Detection of Potential Virulence Genes in Campylobacter jejuni and Campylobacter coli Isolates from Different Sources in Poland

被引：30

作者：

Krutkiewicz, A. ^{[1
]}

Klimuszko, D. ^{[1
]}

机构：

[1] Warsaw Univ Life Sci, Fac Vet Med, Dept Preclin Sci, PL-02786 Warsaw, Poland

来源：

FOLIA MICROBIOLOGICA | 2010年 / 55卷 / 02期

关键词：

FIELD GEL-ELECTROPHORESIS; FLAGELLIN; POULTRY; STRAINS; HUMANS; UPSALIENSIS; PREVALENCE; SEQUENCE; PLASMID; CDTC;

D O I：

10.1007/s12223-010-0025-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was detected by PCR. High prevalence of five different putative virulence and toxin genes (flaA, cdtA, cdtB, cdtC, and cj0588) was found among isolates obtained from children, chickens and dogs. The occurrence of these genes among isolates obtained from pigs was significantly different than for strains isolated from other sources. Two methods for genotyping Campylobacter spp. strains were applied - flaA-typing, and ADSRRS-fingerprinting method, which was used for the first time for Campylobacter spp. strains. Similarity of the genetic profiles was demonstrated in strains isolated from chickens and dogs, and in isolates from chickens and children. Strains isolated from pigs, both C. jejuni as well as C. coli, did not group with isolates from other sources.

引用

页码：167 / 175

页数：9

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