Oral immunization of mice against Clostridium perfringens epsilon toxin with a Lactobacillus casei vector vaccine expressing epsilon toxoid

被引：29
|
作者：
Alimolaei, Mojtaba [1 ,2 ]
Golchin, Mehdi [1 ]
Daneshvar, Hamid [3 ]
机构：
[1] Shahid Bahonar Univ Kerman, Dept Pathobiol, Fac Vet Med, Kerman, Iran
[2] Razi Vaccine & Serum Res Inst, Dept Anaerob Bacterial Vaccine Res & Prod, Kerman Branch, Kerman, Iran
[3] Kerman Univ, Dept Immunol, Sch Med, Kerman, Iran
来源：
INFECTION GENETICS AND EVOLUTION | 2016年 / 40卷
基金：
美国国家科学基金会;
关键词：
Clostridium perfringens; Lactobacillus casei; Vaccine; Toxoid; Enterotoxemia; LACTIC-ACID BACTERIA; NECROTIC ENTERITIS; NEUTRALIZING ANTIBODIES; NASAL IMMUNIZATION; PROTECTS MICE; IMMUNE-SYSTEM; TETANUS TOXIN; ALPHA-TOXIN; MUCOSAL; INDUCTION;
D O I：
10.1016/j.meegid.2016.03.020
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Clostridium perfringens type D infects ruminants and causes the enterotoxemia disease by epsilon-toxin. A mutated epsilon-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-epsilon recombinant strain. BALB/c mice, immunized orally with this strain, highly induced mucosal, humoral, and cell-mediated immune responses and developed a protection against 200 MLD/ml of the activated epsilon-toxin. This study showed that the LC-pT1NX-epsilon could be a promising vaccine candidate against the enterotoxemia disease. (c) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：282 / 287
页数：6
相关论文
共 50 条
  • [21] Clostridium perfringens Epsilon Toxin Increases the Small Intestinal Permeability in Mice and Rats
    Goldstein, Jorge
    Morris, Winston E.
    Fabian Loidl, Cesar
    Tironi-Farinatti, Carla
    McClane, Bruce A.
    Uzal, Francisco A.
    Fernandez Miyakawa, Mariano E.
    PLOS ONE, 2009, 4 (09):
  • [22] Protection efficacy and immunogenicity of Clostridium chauvoei proteins as a subunit blackleg vaccine or an adjuvant for Clostridium perfringens epsilon toxoid
    Hamzavipour, Roxana
    Zahmatkesh, Azadeh
    Paradise, Alireza
    Hosseini, Farzaneh
    TOXICON, 2024, 251
  • [23] Clostridium perfringens Epsilon Toxin Mutant I51C as a Recombinant Vaccine Candidate Against Enterotoxemia
    Nakhaipour, Elmira
    Kariminik, Ashraf
    Alimolaei, Mojtaba
    Bafti, Mehrdad S.
    CLINICAL LABORATORY, 2024, 70 (02) : 296 - 304
  • [24] MATERNALLY INDUCED PROTECTION OF YOUNG LAMBS AGAINST THE EPSILON TOXIN OF CLOSTRIDIUM-PERFRINGENS USING NONACTIVATED VACCINE
    SMITH, LD
    MATSUOKA, T
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1959, 20 (74) : 91 - 93
  • [25] Recombinant Lactobacillus casei expressing Clostridium perfringens toxoids α, β2, ε and β1 gives protection against Clostridium perfringens in rabbits
    Zhao, Li
    Guo, Zhihou
    Liu, Jiali
    Wang, Zi
    Wang, Ruichong
    Li, Yijing
    Wang, Li
    Xu, Yigang
    Tang, Lijie
    Qiao, Xinyuan
    VACCINE, 2017, 35 (32) : 4010 - 4021
  • [26] Protection of goats against experimental enterotoxaemia by vaccination with Clostridium perfringens type D epsilon toxoid
    Uzal, FA
    Kelly, WR
    VETERINARY RECORD, 1998, 142 (26) : 722 - 725
  • [27] Immunization with recombinant fusion of LTB and linear epitope (40-62) of epsilon toxin elicits protective immune response against the epsilon toxin of Clostridium perfringens type D
    Kaushik, Himani
    Deshmukh, Sachin Kumar
    Solanki, Amit Kumar
    Bhatia, Bharti
    Tiwari, Archana
    Garg, Lalit C.
    AMB EXPRESS, 2019, 9 (1)
  • [28] Production and characterization of monoclonal antibodies against Clostridium perfringens Type D epsilon toxin
    Freitas, Theonys Diogenes
    Dias Heneine, Luis Guilherme
    Salvarani, Felipe Masiero
    Silveira Silva, Rodrigo Otavio
    Rocha Dourado Lima, Catarina Guimaraes
    Pires, Prhiscylla Sadana
    de Assis, Ronnie Antunes
    Faria Lobato, Francisco Carlos
    CIENCIA RURAL, 2009, 39 (01): : 269 - 271
  • [29] Clostridium perfringens epsilon toxin mutant Y30A-Y196A as a recombinant vaccine candidate against enterotoxemia
    Bokori-Brown, Monika
    Hall, Charlotte A.
    Vance, Charlotte
    da Costa, Sergio P. Fernandes
    Savva, Christos G.
    Naylor, Claire E.
    Cole, Ambrose R.
    Basak, Ajit K.
    Moss, David S.
    Titball, Richard W.
    VACCINE, 2014, 32 (23) : 2682 - 2687
  • [30] Immunization with a novel Clostridium perfringens epsilon toxin mutant rETXY196E-C confers strong protection in mice
    Wenwu Yao
    Jingjing Kang
    Lin Kang
    Shan Gao
    Hao Yang
    Bin Ji
    Ping Li
    Jing Liu
    Wenwen Xin
    Jinglin Wang
    Scientific Reports, 6
12345