Oral immunization of mice against Clostridium perfringens epsilon toxin with a Lactobacillus casei vector vaccine expressing epsilon toxoid

被引：30

作者：

Alimolaei, Mojtaba ^{[1
,2
]}

Golchin, Mehdi ^{[1
]}

Daneshvar, Hamid ^{[3
]}

机构：

[1] Shahid Bahonar Univ Kerman, Dept Pathobiol, Fac Vet Med, Kerman, Iran

[2] Razi Vaccine & Serum Res Inst, Dept Anaerob Bacterial Vaccine Res & Prod, Kerman Branch, Kerman, Iran

[3] Kerman Univ, Dept Immunol, Sch Med, Kerman, Iran

来源：

INFECTION GENETICS AND EVOLUTION | 2016年 / 40卷

基金：

美国国家科学基金会;

关键词：

Clostridium perfringens; Lactobacillus casei; Vaccine; Toxoid; Enterotoxemia; LACTIC-ACID BACTERIA; NECROTIC ENTERITIS; NEUTRALIZING ANTIBODIES; NASAL IMMUNIZATION; PROTECTS MICE; IMMUNE-SYSTEM; TETANUS TOXIN; ALPHA-TOXIN; MUCOSAL; INDUCTION;

D O I：

10.1016/j.meegid.2016.03.020

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Clostridium perfringens type D infects ruminants and causes the enterotoxemia disease by epsilon-toxin. A mutated epsilon-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-epsilon recombinant strain. BALB/c mice, immunized orally with this strain, highly induced mucosal, humoral, and cell-mediated immune responses and developed a protection against 200 MLD/ml of the activated epsilon-toxin. This study showed that the LC-pT1NX-epsilon could be a promising vaccine candidate against the enterotoxemia disease. (c) 2016 Elsevier B.V. All rights reserved.

引用

页码：282 / 287

页数：6

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