ASSESSMENT OF CYTO/GENOTOXICITY OF IRINOTECAN IN V79 CELLS USING THE COMET, MICRONUCLEUS, AND CHROMOSOME ABERRATION ASSAY

被引：8

作者：

Kasuba, Vilena ^{[1
]}

Rozgaj, Ruzica

Gamulin, Marija ^{[2
]}

Trosic, Ivancica

机构：

[1] Univ Zagreb, Clin Hosp Ctr Zagreb, Mutagenesis Unit, Inst Med Res & Occupat Hlth, HR-10001 Zagreb, Croatia

[2] Univ Zagreb, Oncol Clin, Zagreb, Croatia

来源：

ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY | 2010年 / 61卷 / 01期

关键词：

alkaline comet assay; antineoplastic drug; cell cultures; DNA and cytogenetic damage; topoisomerase; DNA TOPOISOMERASE-I; INTESTINAL DAMAGE; ANTICANCER DRUGS; BREAST-CANCER; STRAND-BREAKS; BLOOD-CELLS; BONE-MARROW; CAMPTOTHECIN; APOPTOSIS; VITRO;

D O I：

10.2478/10004-1254-61-2010-1967

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 mu g mL(-1)), recommended monotherapy dose (9 mu g mL(-1)), and recommended combined therapy dose (4.5 mu g mL(-1)) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test. The cells were treated with irinotecan for 2 h or 24 h. The statistical significance of the results was determined using the one-way ANOVA test and a nonparametric Mann Whitney U test. The comet assay did not show dose-dependent or time-dependent effects. The chromosome aberration analysis showed large DNA rearrangements, i.e., chromosome exchanges. Although the exposed cultures showed a significant increase in micronucleated cells in respect to control, no dose-dependent relation was established among the treated cultures. Time-dependent effect was also not observed.

引用

页码：1 / 9

页数：9

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