Zoonotic Fecal Pathogens and Antimicrobial Resistance in Canadian Petting Zoos

被引：20

作者：

Conrad, Cheyenne C. ^{[1
,2
]}

Stanford, Kim ^{[1
]}

Narvaez-Bravo, Claudia ^{[3
]}

Neumann, Norman F. ^{[4
]}

Munns, Krysty ^{[5
]}

Tymensen, Lisa ^{[1
]}

Jokinen, Cassandra ^{[1
]}

McAllister, Tim A. ^{[5
]}

机构：

[1] Alberta Agr & Forestry, Lethbridge, AB T1J 4V6, Canada

[2] Canadian Assoc Fairs & Exhibit, Brandon, MB R7B 3W8, Canada

[3] Univ Manitoba, Dept Food Sci, Winnipeg, MB R3T 2N2, Canada

[4] Univ Alberta, Dept Publ Hlth, Edmonton, AB T6G 2G7, Canada

[5] Agr & Agri Food Canada, Lethbridge Res & Dev Ctr, Lethbridge, AB T1J 4B1, Canada

来源：

MICROORGANISMS | 2018年 / 6卷 / 03期

关键词：

petting zoo; zoonoses; pathogens; Shiga toxin-producing Escherichia coli (STEC); methicillin-resistant Staphylococcus aureus (MRSA); antimicrobial resistance; ESCHERICHIA-COLI O157; STAPHYLOCOCCUS-AUREUS; CAMPYLOBACTER-JEJUNI; BETA-LACTAMASES; ANIMAL CONTACT; PUBLIC-HEALTH; HAND HYGIENE; WATER; PCR; SALMONELLA;

D O I：

10.3390/microorganisms6030070

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study aimed to better understand the potential public health risk associated with zoonotic pathogens in agricultural fairs and petting zoos in Canada. Prevalence of Salmonella, Shiga toxin-producing Escherichia coli (STEC) O157:H7, and top six non-O157 STEC serogroups in feces (n = 88), hide/feather (n = 36), and hand rail samples (n = 46) was assessed, as well as distributions of antimicrobial resistant (AMR) broad and extended-spectrum beta-lactamase (ESBL)-producing E. coli. Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in pig nasal swabs (n = 4), and Campylobacter, Cryptosporidium, and Giardia in feces was also assessed. Neither Salmonella nor MRSA were detected. Campylobacter spp. were isolated from 32% of fecal samples. Cryptosporidium and Giardia were detected in 2% and 15% of fecal samples, respectively. Only one fecal sample was positive for STEC O157, whereas 22% were positive for non-O157 STEC. Multi-drug resistance (MDR) to antibiotics classified as critically and highly important in human medicine was proportionally greatest in E. coli from cattle feces. The beta-lactamase-producing E. coli from pig, horse/donkey feces, and hand rail samples, as well as the STEC E. coli from handrail swabs were MDR. The diversity and prevalence of zoonotic pathogens and AMR bacteria detected within agricultural fairs and petting zoos emphasize the importance of hygienic practices and sanitization with respect to reducing associated zoonotic risks.

引用

页数：22

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