Production of a novel raw-starch-digesting glucoamylase by Penicillium sp X-1 under solid state fermentation and its use in direct hydrolysis of raw starch

被引：20
|
作者：
Sun, Haiyan
Ge, Xiangyang
Zhang, Weiguo
机构：
[1] So Yangtze Univ, Sch Biotechnol, Wuxi 214036, Jiangsu, Peoples R China
[2] So Yangtze Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214036, Jiangsu, Peoples R China
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2007年 / 23卷 / 05期
关键词：
Penicillium sp; pH stable; raw-starch-digesting glucoamylase; solid state fermentation; synergism;
D O I：
10.1007/s11274-006-9269-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Penicillium sp. X-1, isolated from decayed raw corn, produced high level of raw-starch-digesting glucoamylase (RSDG) under solid state fermentation (SSF). Maximum enzyme yield of 306.2 U g(-1) dry mouldy bran (DMB) was obtained after 36 h of culture upon optimized production. The enzyme could hydrolyse both small and large granule starches but did not adsorb on raw starch. The enzyme exhibited maximum activity at 65 degrees C and pH 6.5, which provided an opportunity of synergism with alpha-amylase. It significantly hydrolysed 15% (w/v) raw corn starch slurry in synergism with the commercial alpha-amylase and a degree of hydrolysis of 92.4% was obtained after 2 h of incubation.
引用
收藏
页码：603 / 613
页数：11
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