Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice

被引：23

作者：

Huang, Chao ^{[1
,2
,3
]}

Wang, Jian ^{[1
,2
,3
]}

Zheng, Xiaodong ^{[1
,2
,3
]}

Chen, Yongyan ^{[1
,2
,3
]}

Zhou, Rongbin ^{[1
,2
,3
,4
]}

Wei, Haiming ^{[1
,2
,3
,4
]}

Sun, Rui ^{[1
,2
,3
,4
]}

Tian, Zhigang ^{[1
,2
,3
,4
]}

机构：

[1] Univ Sci & Technol China, Inst Immunol, Hefei 230027, Anhui, Peoples R China

[2] Univ Sci & Technol China, Sch Life Sci, CAS Key Lab Innate Immun & Chron Dis, Hefei 230027, Anhui, Peoples R China

[3] Univ Sci & Technol China, Med Ctr, Hefei 230027, Anhui, Peoples R China

[4] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China

来源：

JOURNAL OF AUTOIMMUNITY | 2018年 / 93卷

基金：

中国国家自然科学基金;

关键词：

Allergic asthma; Commensal bacteria; NLRP3/IL-1 beta signaling; Post-weaning mice; EARLY-LIFE; AIRWAY INFLAMMATION; NLRP3; INFLAMMASOME; HYGIENE HYPOTHESIS; IMMUNE-SYSTEM; COLONIZATION; MICROBIOTA; MACROPHAGES; SUPPRESSION; DYSBIOSIS;

D O I：

10.1016/j.jaut.2018.07.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Perturbation of commensal bacteria by antibiotic exposure aggravates ovalbumin (OVA)-induced allergic asthma in pre-weaning mice. However, the influence of dysbiosis of commensal bacteria on asthma development in post weaning mice is still limited. Here, we treated 3-week-old post-weaning mice with antibiotics to disrupt corn mensal bacteria and then established OVA-induced allergic asthma by peritoneal sensitization using OVA/alum and intranasal challenge with OVA. Contrary to the protective function in pre-weaning mice, commensal bacteria in post-weaning mice aggravated OVA-induced asthma. Commensal bacteria in post-weaning mice promoted OVA-induced allergic asthma through maintenance of NLRP3/IL-1 beta expression in peritoneal macrophages (pM phi), which promoted recruitment of inflammatory cells, especially inflammatory monocytes, into the peritoneal cavity after OVA/alum sensitization. Further study showed that metronidazole- and vancomycin-sensitive bacteria are involved in maintenance of NLRP3/IL-1 beta signal in pM phi. Our results suggest that certain species of commensal bacteria in post-weaning mice aggravate OVA-induced allergic asthma through NLRP3/IL-1 beta signal pathway.

引用

页码：104 / 113

页数：10