Metabolic network analysis reveals microbial community interactions in anammox granules

被引:620
作者
Lawson, Christopher E. [1 ]
Wu, Sha [2 ]
Bhattacharjee, Ananda S. [2 ]
Hamilton, Joshua J. [3 ]
McMahon, Katherine D. [1 ,3 ]
Goel, Ramesh [2 ]
Noguera, Daniel R. [1 ,4 ]
机构
[1] Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA
[2] Univ Utah, Dept Civil & Environm Engn, Salt Lake City, UT 84112 USA
[3] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[4] Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Wisconsin Energy Inst, Madison, WI 53726 USA
基金
美国国家科学基金会; 加拿大自然科学与工程研究理事会;
关键词
HYDROXYLAMINE OXIDOREDUCTASE; OXIDATION ANAMMOX; SEWAGE-TREATMENT; BACTERIUM; GENOMES; METAPATHWAYS; NITRITATION; DATABASE; REMOVAL; PATHWAY;
D O I
10.1038/ncomms15416
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Microbial communities mediating anaerobic ammonium oxidation (anammox) represent one of the most energy-efficient environmental biotechnologies for nitrogen removal from wastewater. However, little is known about the functional role heterotrophic bacteria play in anammox granules. Here, we use genome-centric metagenomics to recover 17 draft genomes of anammox and heterotrophic bacteria from a laboratory-scale anammox bioreactor. We combine metabolic network reconstruction with metatranscriptomics to examine the gene expression of anammox and heterotrophic bacteria and to identify their potential interactions. We find that Chlorobi-affiliated bacteria may be highly active protein degraders, catabolizing extracellular peptides while recycling nitrate to nitrite. Other heterotrophs may also contribute to scavenging of detritus and peptides produced by anammox bacteria, and potentially use alternative electron donors, such as H2, acetate and formate. Our findings improve the understanding of metabolic activities and interactions between anammox and heterotrophic bacteria and offer the first transcriptional insights on ecosystem function in anammox granules.
引用
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页数:12
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