DNA Barcoding Techniques for Avian Influenza Virus Surveillance in Migratory Bird Habitats

被引：60

作者：

Lee, Don-Hun ^{[1
]}

Lee, Hyun-Jeong ^{[1
]}

Lee, Youn-Jeong ^{[2
]}

Kang, Hyun-Mi ^{[2
]}

Jeong, Ok-Mi ^{[2
]}

Kim, Min-Chul ^{[2
]}

Kwon, Ji-Sun ^{[2
]}

Kwon, Jun-Hun ^{[2
]}

Kim, Chang-Bae ^{[3
]}

Lee, Joong-Bok ^{[1
]}

Park, Seung-Yong ^{[1
]}

Choi, In-Soo ^{[1
]}

Song, Chang-Seon ^{[1
]}

机构：

[1] Konkuk Univ, Coll Vet Med, Avian Dis Lab, Seoul 143701, South Korea

[2] Natl Vet Res & Quarantine Serv, Avian Dis Div, Anyang Si 430824, Gyeonggi Do, South Korea

[3] Sangmyung Univ, Dept Life Sci, Seoul 110743, South Korea

来源：

JOURNAL OF WILDLIFE DISEASES | 2010年 / 46卷 / 02期

关键词：

Avian influenza virus; DNA barcoding; fecal sample; surveillance; PCR;

D O I：

10.7589/0090-3558-46.2.649

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Avian influenza virus (AIV) circulates among free-ranging, wild birds. We optimized and validated a DNA barcoding technique for AIV isolation and host-species identification using fecal samples from wild birds. DNA barcoding was optimized using tissue and fecal samples from known bird species, and the method was shown to distinguish 26 bird species. Subsequently, fecal samples (n=743) collected from wild waterfowl habitats confirmed the findings from the laboratory tests. All identified AIV-positive hosts (n=35) were members of the order Anseriformes. We successfully applied the DNA barcoding technique to AIV surveillance and examined ATV epidemiology and host ecology in these wild waterfowl populations. This methodology may be useful in the design of AN surveillance strategies.

引用

页码：649 / 654

页数：6

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