Improvement of the Representation of Bifidobacteria in Fecal Microbiota Metagenomic Libraries by Application of the cpn60 Universal Primer Cocktail

被引：35

作者：

Hill, Janet E. ^{[1
]}

Fernando, W. M. Ursla ^{[2
]}

Zello, Gordon A. ^{[3
]}

Tyler, Robert T. ^{[4
]}

Dahl, Wendy J. ^{[3
,5
]}

Van Kessel, Andrew G. ^{[2
]}

机构：

[1] Univ Saskatchewan, Dept Vet Microbiol, Saskatoon, SK S7N 5B4, Canada

[2] Univ Saskatchewan, Dept Anim & Poultry Sci, Saskatoon, SK S7N 5B4, Canada

[3] Univ Saskatchewan, Div Nutr & Dietet, Saskatoon, SK S7N 5B4, Canada

[4] Univ Saskatchewan, Dept Food & Bioprod Sci, Saskatoon, SK S7N 5B4, Canada

[5] Univ Florida, Dept Food Sci & Human Nutr, Gainesville, FL 32611 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 13期

关键词：

HUMAN INTESTINAL MICROBIOTA; SEQUENCE-BASED METHODS; QUANTITATIVE PCR; PREDOMINANT BACTERIA; GENUS CLOSTRIDIUM; HUMAN FECES; SAMPLES; QUANTIFICATION; COMMUNITIES; POPULATION;

D O I：

10.1128/AEM.01510-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Actinobacteria, particularly bifidobacteria, are widely observed to be underrepresented in metagenomic studies of microbial communities. We have compared human fecal microbiota clone libraries based on 16S rRNA and cpn60 PCR products. Taxonomic profiles were similar except that the cpn60 libraries contained large numbers of bifidobacterial sequences.

引用

页码：4550 / 4552

页数：3

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