Assessing patterns for compressive fluorescence lifetime imaging

被引:20
作者
Ochoa, M. [1 ]
Pian, O. [1 ]
Yao, R. [1 ]
Ducros, N. [2 ]
Intes, X. [1 ]
机构
[1] Rensselaer Polytech Inst, Dept Biomed Engn, Troy, NY 12180 USA
[2] UCB Lyon 1, INSA Lyon, Univ Lyon, UJM St Etienne,CNRS,INSERM,CREATIS UMR 5220,U1206, F-69621 Lyon, France
基金
美国国家卫生研究院;
关键词
MICROSCOPY;
D O I
10.1364/OL.43.004370
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A novel hyperspectral single pixel system was used to compare different compressive basis patterns for intensity imaging, lifetime imaging, and FRET quantification. Six popular basis patterns were compared experimentally in a phantom containing two fluorescent dyes. The basis patterns that performed best for lifetime quantification were used to measure FRET occurrence in well-plate samples with varying acceptor-donor ratios. The ABS-WP approach using Haar patterns and the compressive sensing approach with Hadamard Ranked patterns displayed the best overall performances at a 50% compression ratio. (C) 2018 Optical Society of America.
引用
收藏
页码:4370 / 4373
页数:4
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