Characterization of new metabolites from in vivo biotransformation of norisoboldine by liquid chromatography/mass spectrometry and NMR spectroscopy

被引：25

作者：

Chen, Jian-Zhong ^{[1
]}

Chou, Gui-Xin ^{[2
,3
,4
]}

Wang, Chang-Hong ^{[2
,3
]}

Yang, Li ^{[2
,3
]}

Bligh, S. W. Annie ^{[5
]}

Wang, Zheng-Tao ^{[1
,2
,3
,4
]}

机构：

[1] China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210038, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, MOE Key Lab Standardizat Chinese Med, Inst Chinese Mat Med, Shanghai 201210, Peoples R China

[3] Shanghai Univ Tradit Chinese Med, SATCM Key Lab New Resources & Qual Evaluat Chines, Inst Chinese Mat Med, Shanghai 201210, Peoples R China

[4] Shanghai R&D Ctr Standardizat Chinese Med, Shanghai 201210, Peoples R China

[5] London Metropolitan Univ, Inst Hlth Res & Policy, London N7 8DB, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 52卷 / 05期

关键词：

Norisoboldine; Metabolite; Identification; Ion-trap; NMR; MASS-SPECTROMETRY; LINDERA-AGGREGATA; ALKALOIDS; IDENTIFICATION; ROOT;

D O I：

10.1016/j.jpba.2010.02.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nolisoboldine (1,9-dihydroxy-2,10-dimethoxynoraporphine) is one of the major bioactive isoquinoline alkaloids in Linderae Radix, a commonly used Chinese herbal medicine The aim of this study is to isolate and characterize metabolites of norisoboldine after gavage feeding in rats High-performance liquid chromatography coupled with electrospray ionization and ion-trap mass spectrometry (HPLC-ESI/MS") was used to identify metabolites of norisoboldine in rat urine and bile samples A total of five metabolites of norisoboldine were characterized by comparing retention time and UV absorption in HPLC, and by molecular mass and fragmentation pattern of the analytes by mass spectrometry with those of norisoboldine Two new glucuronide conjugates of norisoboldine, noriosboldine-1-O-beta-D-glucuionide and rim isobolchne-9-O-alpha-D-glucuromde, were isolated from rat urine samples and their structures were ;confirmed by NMR spectioscopy ((1H), C-13, HMBC and HSQC) for the fiist time The iesults suggested that glucuromdation and sulfation were involved in metabolic pathways of nonsobolchne in rat (C) 2010 Elsevier B V All tights ieseived

引用

页码：687 / 693

页数：7

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