Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome

被引：773

作者：

Carrion, Victor J. ^{[1
,2
]}

Perez-Jaramillo, Juan ^{[1
,3
]}

Cordovez, Viviane ^{[1
,2
]}

Tracanna, Vittorio ^{[4
]}

de Hollander, Mattias ^{[1
]}

Ruiz-Buck, Daniel ^{[1
]}

Mendes, Lucas W. ^{[5
]}

van Ijcken, Wilfred F. J. ^{[6
]}

Gomez-Exposito, Ruth ^{[1
,7
]}

Elsayed, Somayah S. ^{[2
]}

Mohanraju, Prarthana ^{[7
]}

Arifah, Adini ^{[7
]}

van der Oost, John ^{[7
]}

Paulson, Joseph N. ^{[8
]}

Mendes, Rodrigo ^{[9
]}

van Wezel, Gilles P. ^{[1
,2
]}

Medema, Marnix H. ^{[4
]}

Raaijmakers, Jos M. ^{[1
,2
]}

机构：

[1] Netherlands Inst Ecol NIOO KNAW, Dept Microbial Ecol, Droevendaalsesteeg 10, NL-6708 PB Wageningen, Netherlands

[2] Leiden Univ, Inst Biol, Sylviusweg 72, NL-2333 BE Leiden, Netherlands

[3] Univ Antioquia, PECET, Medellin 050010, Antioquia, Colombia

[4] Wageningen Univ, Bioinformat Grp, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands

[5] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Cell & Mol Biol Lab, Piracicaba, Brazil

[6] Univ Med Ctr Rotterdam, Erasmus MC, Ctr Biom, Dept Cell Biol, NL-3025 CN Rotterdam, Netherlands

[7] Wageningen Univ & Res, Lab Microbiol, Stippeneng 4, NL-6708 WE Wageningen, Netherlands

[8] Genentech Inc, Dept Biostat Prod Dev, San Francisco, CA 94080 USA

[9] Brazilian Agr Res Corp, Lab Environm Microbiol, Embrapa Environm, Rodovia SP 340,Km 127-5, BR-13820000 Jaguariuna, Brazil

来源：

SCIENCE | 2019年 / 366卷 / 6465期

关键词：

RHIZOSPHERE MICROBIOME; BACTERIAL COMMUNITIES; SOIL MICROBIOMES; READ ALIGNMENT; PLANT; GENE; FLAVOBACTERIUM; DATABASE; CYTOPHAGA; MANIPULATION;

D O I：

10.1126/science.aaw9285

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Microorganisms living inside plants can promote plant growth and health, but their genomic and functional diversity remain largely elusive. Here, metagenomics and network inference show that fungal infection of plant roots enriched for Chitinophagaceae and Flavobacteriaceae in the root endosphere and for chitinase genes and various unknown biosynthetic gene clusters encoding the production of nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). After strain-level genome reconstruction, a consortium of Chitinophaga and Flavobacterium was designed that consistently suppressed fungal root disease. Site-directed mutagenesis then revealed that a previously unidentified NRPS-PKS gene cluster from Flavobacterium was essential for disease suppression by the endophytic consortium. Our results highlight that endophytic root microbiomes harbor a wealth of as yet unknown functional traits that, in concert, can protect the plant inside out.

引用

页码：606 / +

页数：74

共 94 条

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