Isolation and molecular characterization of Leptospira borgpetersenii serovar Hardjo strain Hardjobovis in the urine of naturally infected cattle in Brazil

被引：22

作者：

Chideroli, R. T. ^{[1
]}

Pereira, U. P. ^{[2
]}

Goncalves, D. D. ^{[3
]}

Nakamura, A. Y. ^{[4
]}

Alfieri, A. A. ^{[5
]}

Alfieri, A. F. ^{[5
]}

Freitas, J. C. ^{[1
]}

机构：

[1] Univ Estadual Londrina, Dept Med Vet Prevent, Lab Leptospirose, Londrina, PR, Brazil

[2] Univ Estadual Londrina, Dept Med Vet Prevent, Lab Bacteriol, Londrina, PR, Brazil

[3] Univ Paranaense, Lab Med Vet Prevent & Saude Publ, Umuarama, PR, Brazil

[4] Univ Estadual Maringa, Med Vet, Umuarama, PR, Brazil

[5] Univ Estadual Londrina, Dept Med Vet Prevent, Lab Virol Anim, Londrina, PR, Brazil

来源：

GENETICS AND MOLECULAR RESEARCH | 2016年 / 15卷 / 01期

关键词：

Bovine leptospirosis; MLVA; Diagnosis; Bacterial culture; RISK-FACTORS; IDENTIFICATION; VACCINATION; PARANA; STATE; BOVIS; SPP;

D O I：

10.4238/gmr.15018473

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hardjobovis by molecular characterization. Fifteen dairy cows with a history of reproductive failure, such as abortion and infertility, were selected. Urine samples obtained from each animal were immediately seeded in tubes containing Ellinghausen-McCullough-Johnson-Harris culture medium. The identification of the isolates was performed by Multilocus variable-number tandem-repeat analysis (MLVA) technique and phylogenetic analysis of partial sequence of gene sec Y. From the 15 urine samples evaluated, two Leptospira were found and identified as the Londrina 49 and Londrina 54 strains. The MLVA profiles and sequencing of gene sec Y characterized the isolates as L. borgpetersenii serovar Hardjo strain Hadjobovis because it has different genetic pattern of Leptospira interrogans serovar Hardjo strain Hardjoprajitno. Therefore, more studies are needed including isolation and molecular characterization from regional strains to obtain a better knowledge about epidemiology of serovar Hardjo in bovine which may assist in future strategies of prevention and control of bovine leptospirosis.

引用

页数：7

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