The rice transcription factor Nhd1 regulates root growth and nitrogen uptake by activating nitrogen transporters

被引：32

作者：

Li, Kangning ^{[1
]}

Zhang, Shunan ^{[1
]}

Tang, Shuo ^{[1
]}

Zhang, Jun ^{[1
]}

Dong, Hongzhang ^{[1
]}

Yang, Shihan ^{[1
]}

Qu, Hongye ^{[1
]}

Xuan, Wei ^{[1
]}

Gu, Mian ^{[1
]}

Xu, Guohua ^{[1
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Key Lab Plant Nutr & Fertilizat Low Middle Reache, Minist Agr, Nanjing 210095, Peoples R China

来源：

PLANT PHYSIOLOGY | 2022年 / 189卷 / 03期

基金：

中国国家自然科学基金;

关键词：

FLOWERING TIME REGULATION; AFFINITY AMMONIUM UPTAKE; USE EFFICIENCY; NITRATE TRANSPORTERS; ARABIDOPSIS ROOTS; EXPRESSION; NITRIFICATION; RESPONSES; YIELD; RHIZOSPHERE;

D O I：

10.1093/plphys/kiac178

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plants adjust root architecture and nitrogen (N) transporter activity to meet the variable N demand, but their integrated regulatory mechanism remains unclear. We have previously reported that a floral factor in rice (Oryza sativa), N-mediated heading date-1 (Nhd1), regulates flowering time. Here, we show that Nhd1 can directly activate the transcription of the high-affinity ammonium (NH4+) transporter 1;3 (OsAMT1;3) and the dual affinity nitrate (NO3-) transporter 2.4 (OsNRT2.4). Knockout of Nhd1 inhibited root growth in the presence of NO3- or a low concentration of NH4+. Compared to the wild-type (WT), nhdl and osamt1;3 mutants showed a similar decrease in root growth and N uptake under low NH4+ supply, while nhdl and osnrt2.4 mutants showed comparable root inhibition and altered NO3- translocation in shoots. The defects of nhdl mutants in NH4+ uptake and root growth response to various N supplies were restored by overexpression of OsAMT1;3 or OsNRT2.4. However, when grown in a paddy field with low N availability, nhdl mutants accumulated more N and achieved a higher N uptake efficiency (NUpE) due to the delayed flowering time and prolonged growth period. Our findings reveal a molecular mechanism underlying the growth duration-dependent NUpE.

引用

页码：1608 / 1624

页数：17

共 89 条

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