Selection strategies for improved biocatalysts

被引：40
作者：
Boersma, Ykelien L.
Droge, Melloney J.
Quax, Wim J.
机构：
[1] Univ Groningen, Inst Drug Explorat, Dept Pharmaceut Biol, NL-9713 AV Groningen, Netherlands
[2] ABL BV, Assen, Netherlands
来源：
FEBS JOURNAL | 2007年 / 274卷 / 09期
关键词：
cell surface display; directed evolution; enzymes; genetic selection; in vitro compartmentalization; phage display; ribosome display;
D O I：
10.1111/j.1742-4658.2007.05782.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However, their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adapting the characteristics of an enzyme. However, selection of the evolved variants is a critical step, and therefore new strategies to enable selection of the desired enzymatic activity have been developed. This review focuses on these novel strategies for selecting enzymes from large libraries, in particular those that are used in the synthesis of pharmaceutical intermediates and pharmaceuticals.
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收藏
页码：2181 / 2195
页数：15
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